Betulin isolated from Pyrola incarnata Fisch. inhibited lipopolysaccharide (LPS)-induced neuroinflammation with the guidance of computer-aided drug design
文献类型：期刊论文
| 作者 | Liu, Qian; Liu, Jin-Ping; Mei, Jia-Hui; Li, Shuang-Jun; Shi, Li-Qiao; Lin, Zong-Hao; Xie, Bai-Yan; Sun, Wei-Guang; Wang, Zhen-Yu; Yang, Xi-Liang |
| 刊名 | BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
 |
| 出版日期 | 2020 |
| 卷号 | 30期号:12页码:127193 |
| 关键词 | Betulin Lipopolysaccharide Microglia cells Neuroinflammation Pyrola incarnata |
| ISSN号 | 0960-894X |
| DOI | 10.1016/j.bmcl.2020.127193 |
| 英文摘要 | This study aims to investigate active phytochemicals isolated from Pyrola incarnata Fisch. (P. incarnata) and their protection against neuroinflammation induced by LPS. Betulin, accompanied with other 9 compounds, were isolated from P. incarnata and elucidated by spectroscopic analysis (H-1-, C-13 NMR). ELISA kits and the measurement of NO production based on Griess reaction showed that betulin (5) (250 mu g/mL) could suppress LPS-induced activation of microglial cell BV-2 better than others by inhibiting inflammatory cytokines (TNF-alpha, IL-6, IL-1 beta) expression and NO production. With the guidance of computer-aided drug design and the analysis of biological experiment, we demonstrated betulin could reduce LPS-induced iNOS expression, prevent JNKs pathways, and down-regulate the phosphorylation levels of NF-kappa B/p65. In conclusion, betulin isolated from P. incarnata possessed outstanding anti-neuroinflammation potential, presumably related to iNOS expression, JNKs and NF-kappa B/p65 pathways. Therefore, Pyrola incarnata may be a valuable natural resource and betulin is a potential drug for the treatment of neurodegenerative disorders by inhibiting inflammatory mediators. |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31900286]; Scientific Research Projects of Hubei education department for Young Scholars [Q20171109]; Hubei agricultural science and technology innovation center [2016-620-000-001-039] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31900286]; Scientific Research Projects of Hubei education department for Young Scholars [Q20171109]; Hubei agricultural science and technology innovation center [2016-620-000-001-039] |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/18398]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Hubei Acad Agr Sci, Hubei Biopesticide Engn Res Ctr, 8 Nanhu Ave, Wuhan 430064, Peoples R China 2.Wuhan Univ Sci & Technol, Inst Pharmaceut Innovat, Dept Pharm,Med Coll, Hubei Prov Key Lab Occupat Hazard Identificat & C, Wuhan 430081, Peoples R China 3.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Peoples R China 4.Wuhan Inst Drug & Med Device Control, Wuhan 430075, Peoples R China 5.Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Pharm, Hubei Key Lab Nat Med Chem & Resource Evaluat, Wuhan 430030, Peoples R China 6.Wuhan Univ Sci & Technol, Med Coll, Inst Infect Immunol & Tumor Microenvironm, Dept Pharm,Hubei Prov Key Lab Occupat Hazard Iden, Wuhan 430081, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Qian,Liu, Jin-Ping,Mei, Jia-Hui,et al. Betulin isolated from Pyrola incarnata Fisch. inhibited lipopolysaccharide (LPS)-induced neuroinflammation with the guidance of computer-aided drug design[J]. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS,2020,30(12):127193. |
| APA | Liu, Qian.,Liu, Jin-Ping.,Mei, Jia-Hui.,Li, Shuang-Jun.,Shi, Li-Qiao.,...&Fang, Wei.(2020).Betulin isolated from Pyrola incarnata Fisch. inhibited lipopolysaccharide (LPS)-induced neuroinflammation with the guidance of computer-aided drug design.BIOORGANIC & MEDICINAL CHEMISTRY LETTERS,30(12),127193. |
| MLA | Liu, Qian,et al."Betulin isolated from Pyrola incarnata Fisch. inhibited lipopolysaccharide (LPS)-induced neuroinflammation with the guidance of computer-aided drug design".BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 30.12(2020):127193. |
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来源：南海海洋研究所
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