Autophagy Dually Induced by AMP Surplus and Oxidative Stress Enhances Hemocyte Survival and Bactericidal Capacity via AMPK Pathway inCrassostrea hongkongensis
文献类型：期刊论文
| 作者 | Dang, Xin3,4; Wong, Nai-Kei; Xie, Yongli5; Thiyagarajan, Vengatesen3; Mao, Fan1,4; Zhang, Xiangyu1,4; Lin, Yue1,4; Xiang, Zhiming1,4; Li, Jun1,4; Xiao, Shu1,4 |
| 刊名 | FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
 |
| 出版日期 | 2020 |
| 卷号 | 8页码:411 |
| ISSN号 | 2296-634X |
| 关键词 | AMPK phosphorylation apoptosis autophagy Hong Kong oyster infection ROS Vibrio parahaemolyticus |
| DOI | 10.3389/fcell.2020.00411 |
| 英文摘要 | Crassostrea hongkongensis(Hong Kong oyster) is an ecologically and economically valuable shellfish endemic to South/Southeast Asia. Due to ocean acidification and warming waters, they have become increasingly vulnerable to invading microbes includingVibrio parahaemolyticus, a significant foodborne human pathogen. In recent years, outbreaks ofV. parahaemolyticushave emerged as a perennial phenomenon in parts of the world, necessitating to better understand the biology of host-pathogen interactions in this under-examined marine invertebrate. Although an immunologically relevant autophagy apparatus has been identified inCrassostrea gigas, an evolutionarily close mollusk cousin, the precise mechanistic details ofC. hongkongensisautophagy duringV. parahaemolyticusinfection are still wanting. Here, we compellingly demonstrated thatin vivo V. parahaemolyticuschallenge robustly triggered autophagic signaling inC. hongkongensishemocytes peaking at 6 h post-infection, which subsequently promoted bacterial clearance and dampened premature apoptosis. Simultaneously, a large surplus of adenosine monophosphate (AMP) and elevations in reactive oxygen species (ROS, specifically mitochondrial O(2)(-)and cellular H2O2) formation were observed post-infection. Extrinsically applied AMP and ROS could synergistically induce AMP-activated protein kinase (AMPK) phosphorylation to stimulate downstream autophagic events.V. parahaemolyticusinfection-induced autophagy was pharmacologically shown to be AMPK-dependentin vivo. Overall, our results establish autophagy as a crucial arm of host defense againstVibrioinfections in mollusks, and provide new insights into the underappreciated roles of ROS and AMP as co-regulators of autophagy. |
| 资助机构 | Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) [GML2019ZD0407]; National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31902404]; China Agricultural Research System [CARS-49]; Science and Technology Program of Guangzhou, China [201804020073]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [201806010003]; Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences [ISEE2018PY01, ISEE2018PY03, ISEE2018ZD01]; Science and Technology Planning Project of Guangdong Province, China [2017B030314052, 201707010177] ; Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) [GML2019ZD0407]; National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31902404]; China Agricultural Research System [CARS-49]; Science and Technology Program of Guangzhou, China [201804020073]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [201806010003]; Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences [ISEE2018PY01, ISEE2018PY03, ISEE2018ZD01]; Science and Technology Planning Project of Guangdong Province, China [2017B030314052, 201707010177] |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/18402]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Southern Univ Sci & Technol, Natl Clin Res Ctr Infect Dis, Shenzhen Third Peoples Hosp, Second Hosp Affiliated, Shenzhen, Peoples R China 2.Chinese Acad Sci, Innovat Acad South China Sea Ecol & Environm Engn, Guangzhou, Peoples R China 3.Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Prov Key Lab Appl Marine Biol, South China Sea Inst Oceanol, Guangzhou, Peoples R China 4.Univ Hong Kong, Swire Inst Marine Sci, Sch Biol Sci, Hong Kong, Peoples R China 5.Southern Marine Sci & Engn Guangdong Lab, Guangzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dang, Xin,Wong, Nai-Kei,Xie, Yongli,et al. Autophagy Dually Induced by AMP Surplus and Oxidative Stress Enhances Hemocyte Survival and Bactericidal Capacity via AMPK Pathway inCrassostrea hongkongensis[J]. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,2020,8:411. |
| APA | Dang, Xin.,Wong, Nai-Kei.,Xie, Yongli.,Thiyagarajan, Vengatesen.,Mao, Fan.,...&Yu, Ziniu.(2020).Autophagy Dually Induced by AMP Surplus and Oxidative Stress Enhances Hemocyte Survival and Bactericidal Capacity via AMPK Pathway inCrassostrea hongkongensis.FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,8,411. |
| MLA | Dang, Xin,et al."Autophagy Dually Induced by AMP Surplus and Oxidative Stress Enhances Hemocyte Survival and Bactericidal Capacity via AMPK Pathway inCrassostrea hongkongensis".FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY 8(2020):411. |
入库方式： OAI收割
来源：南海海洋研究所
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