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Transcriptomic Evidence Reveals the Molecular Basis for Functional Differentiation of Hemocytes in a Marine Invertebrate,Crassostrea gigas

文献类型：期刊论文

作者Mao, Fan1,2,3; Wong, Nai-Kei; Lin, Yue1,2,3; Zhang, Xiangyu1,2,3; Liu, Kunna1,2,3; Huang, Minwei1,2,3; Xu, Duo1,2,3; Xiang, Zhiming1,2,3; Li, Jun1,2,3; Zhang, Yang1,2,3
刊名FRONTIERS IN IMMUNOLOGY
出版日期2020
卷号11页码:911
关键词oyster functional differentiation granulocytes Cdc42 Fos
ISSN号1664-3224
DOI10.3389/fimmu.2020.00911
英文摘要Hemocytes play unequivocally central roles in host immune defense of bivalve mollusks, though the exact mechanisms underlying their functional differentiation are only partially understood. To this end, granulocytes and hyalinocytes were sorted via flow cytometry from hemocytes of the Pacific oysterCrassostrea gigas, and consequently quantitative transcriptomic analysis revealed a striking array of differentially expressed genes (DEGs), which were globally upregulated in granulocytes, dedicating to functional differentiation among oyster hemocytes. Our network of DEGs illustrated actively engaged signaling pathways, with Cdc42/Cdc42l being a core regulator of pathway network, which was validated by a dramatically reduced capacity for hemocyte phagocytosis in the presence of Cdc42 inhibitors. Additionally, a number of transcription factors were identified among DEGs, including ELK, HELT, and Fos, which were predominantly expressed in granulocytes. The AP-1 transcription factor Fos was confirmed to facilitate functional differentiation of hemocytes in an assay on binding to target genes by the AP-1 binding site, consistent with downstream phagocytosis and ROS production. Importantly, Cdc42/Cdc42l were also regulated by the expression of Fos, providing a possible regulatory mechanism-guided hemocyte functional differentiation. Findings in this study have bridged a knowledge gap on the mechanistic underpinnings of functional differentiation of hemocytes in a marine invertebrateC. gigas, which promise to facilitate research on the evolution of immune defense and functional differentiation of phagocyte in higher-order and more recent phyla.
资助机构National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31902404]; Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) [GML2019ZD0407]; China Agricultural Research System [CARS-49]; Science and Technology Program of Guangzhou, China [201804020073]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [201806010003]; Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences [ISEE2018PY01, ISEE2018PY03, ISEE2018ZD01]; Science and Technology Planning Project of Guangdong Province, China [2017B030314052, 201707010177] ; National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31902404]; Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) [GML2019ZD0407]; China Agricultural Research System [CARS-49]; Science and Technology Program of Guangzhou, China [201804020073]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [201806010003]; Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences [ISEE2018PY01, ISEE2018PY03, ISEE2018ZD01]; Science and Technology Planning Project of Guangdong Province, China [2017B030314052, 201707010177]
源URL[http://ir.scsio.ac.cn/handle/344004/18417]  
专题南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
作者单位1.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou, Peoples R China
2.Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou, Peoples R China
3.Southern Marine Sci & Engn Guangdong Lab Guangzho, Guangzhou, Peoples R China
4.Chinese Acad Sci, Innovat Acad South China Sea Ecol & Environm Engn, Guangzhou, Peoples R China
5.Southern Univ Sci & Techno, Shenzhen Peoples Hosp 3, Dept Infect Dis, Hosp 2, Shenzhen, Peoples R China
推荐引用方式
GB/T 7714		 Mao, Fan,Wong, Nai-Kei,Lin, Yue,et al. Transcriptomic Evidence Reveals the Molecular Basis for Functional Differentiation of Hemocytes in a Marine Invertebrate,Crassostrea gigas[J]. FRONTIERS IN IMMUNOLOGY,2020,11:911.
APA Mao, Fan.,Wong, Nai-Kei.,Lin, Yue.,Zhang, Xiangyu.,Liu, Kunna.,...&Yu, Ziniu.(2020).Transcriptomic Evidence Reveals the Molecular Basis for Functional Differentiation of Hemocytes in a Marine Invertebrate,Crassostrea gigas.FRONTIERS IN IMMUNOLOGY,11,911.
MLA Mao, Fan,et al."Transcriptomic Evidence Reveals the Molecular Basis for Functional Differentiation of Hemocytes in a Marine Invertebrate,Crassostrea gigas".FRONTIERS IN IMMUNOLOGY 11(2020):911.

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来源：南海海洋研究所

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