Hypolipidemic effects of protein hydrolysates fromTrachinotus ovatusand identification of peptides implied in bile acid-binding activity using LC-ESI-Q-TOF-MS/MS
文献类型：期刊论文
| 作者 | Wan, Peng2; Chen, Deke2; Chen, Hua2; Zhu, Xiaolian; Chen, Xin1; Sun, Huili2; Pan, Jianyu2; Cai, Bingna2 |
| 刊名 | RSC ADVANCES
 |
| 出版日期 | 2020 |
| 卷号 | 10期号:34页码:20098 |
| DOI | 10.1039/d0ra02428g |
| 英文摘要 | In the present work, analysis of the hypolipidemic properties ofTrachinotus ovatusprotein hydrolysates (TOPHs) and identification of peptides with bile acid-binding activity were performed. Hydrolysates prepared by trypsin digestion exhibited the highestin vitrobile acid-binding capacities compared with hydrolysates prepared with the other four proteases and were mainly composed of small peptides and amino acids with molecular weights <3 kDa, accounting for 77.30%. Among the five ultra-filtration fractions of TOPHs, TOPHs-5 (<3 kDa) exhibited the highestin vitrobile acid-binding capacity, which was equivalent to 77.97% of cholestyramine at the same concentration. A total of 68 peptides were identified from TOPHs-5 by LC-ESI-Q-TOF-MS/MS and 9 of them had hydrophobicity of more than 60%. These highly hydrophobic peptides might be associated with the bile acid-binding activity of TOPHs-5.In vivoexperiments indicated that the TOPHs could effectively reduce total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and the atherogenic index (AI), while they could evidently increase the high-density lipoprotein cholesterol (HDL-C) content. Furthermore, TOPHs exerted a marked protective effect on hepatorenal function, as evidenced by decreased levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and creatinine (CREA). Histopathological studies confirmed that TOPHs evidently protected the liver from histological alterations. In summary, for the first time, hypolipidemic effects and subsequential identification were obtained from TOPHs, which are promising natural ingredients that could potentially be employed in the management of hyperlipidemia. |
| 资助机构 | National Key R&D Program of China [2018YFC0311202]; Science and Technology Program of Guangzhou, China [201804010321, 201804010364, 201707010164]; Natural Science Foundation of Guangdong ProvinceNational Natural Science Foundation of Guangdong Province [2018A0303130144, 2018A030313903, 2018A030313088, 2018A030313626]; Special Project for Marine Economic Development of Guangdong Province [[2020]036, [2020]038]; Open Projects of Guangdong Key Laboratory of Marine Materia Medica [LMM2016-4]; Pearl River S&T Nova Program of Guangzhou [201710010095] ; National Key R&D Program of China [2018YFC0311202]; Science and Technology Program of Guangzhou, China [201804010321, 201804010364, 201707010164]; Natural Science Foundation of Guangdong ProvinceNational Natural Science Foundation of Guangdong Province [2018A0303130144, 2018A030313903, 2018A030313088, 2018A030313626]; Special Project for Marine Economic Development of Guangdong Province [[2020]036, [2020]038]; Open Projects of Guangdong Key Laboratory of Marine Materia Medica [LMM2016-4]; Pearl River S&T Nova Program of Guangzhou [201710010095] |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/18418]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med, South China Sea Inst Oceanol, Guangzhou 510301, Peoples R China 2.Foshan Univ, Sch Environm & Chem Engn, Foshan, Peoples R China 3.Chinese Acad Sci, Innovat Acad South China Sea Ecol & Environm Engn, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wan, Peng,Chen, Deke,Chen, Hua,et al. Hypolipidemic effects of protein hydrolysates fromTrachinotus ovatusand identification of peptides implied in bile acid-binding activity using LC-ESI-Q-TOF-MS/MS[J]. RSC ADVANCES,2020,10(34):20098, 20109. |
| APA | Wan, Peng.,Chen, Deke.,Chen, Hua.,Zhu, Xiaolian.,Chen, Xin.,...&Cai, Bingna.(2020).Hypolipidemic effects of protein hydrolysates fromTrachinotus ovatusand identification of peptides implied in bile acid-binding activity using LC-ESI-Q-TOF-MS/MS.RSC ADVANCES,10(34),20098. |
| MLA | Wan, Peng,et al."Hypolipidemic effects of protein hydrolysates fromTrachinotus ovatusand identification of peptides implied in bile acid-binding activity using LC-ESI-Q-TOF-MS/MS".RSC ADVANCES 10.34(2020):20098. |
入库方式： OAI收割
来源：南海海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。