Optimization of medium using response surface methodology to enhance the growth of Effrenium voratum (Symbiodiniaceae, Dinophyceae)
文献类型：期刊论文
| 作者 | Yang, Fangfang; Long, Chao; Wei, Zhangliang; Long, Lijuan |
| 刊名 | JOURNAL OF PHYCOLOGY
 |
| 关键词 | Effrenium voratum fatty acid composition growth medium response surface methodology |
| ISSN号 | 0022-3646 |
| DOI | 10.1111/jpy.13007 |
| 英文摘要 | Survival of coral reef-associated Symbiodiniaceae is vital to maintain the healthy coral community in coral reefs. However, knowledge about cultivation of free-living or symbiotic Symbiodiniaceae has been limited. In this study, the response surface methodology was applied to optimize the medium for Effrenium voratum. The results showed that the impacts of nutrient components on algal growth were: FeCl3> NaH2PO4>MnSO4> MgSO4/CoSO4 > KCl>ZnSO4> CaCl2/NaNO3, among which NaH2PO4 and FeCl3 significantly affected algal growth. The optimal medium was: natural seawater supplemented with NaH2PO4 center dot 2H(2)O 0.25 mM,FeCl3 center dot 6H(2)O 14.24 mu M, NaNO3 0.94 mM, MgSO4 center dot 7H(2)O 40.63 mM, KCl 5.37 mM, CaCl2 center dot 2H(2)O 4.08 mM, ZnSO4 center dot 7H(2)O 0.35 mu M, MnSO4 9.93 mu M, and CoSO4 0.36 mu M. The use of the optimized medium resulted in an increase of biomass yield (0.76 g dry weight center dot L-1) by 46% over that using the initial medium, which agreed with the predicted value (0.71 g center dot L-1). Additionally, fatty acids, mainly consisting of palmitic acid (C16:0) and ethyl carbonate (C20:0), accounted for approximately 50% of the total fatty acids in E. voratum. Interestingly, docosahexaenoic acid (DHA) accounted for 6% of total fatty acids, a high proportion that makes E. voratum a potential candidate feedstock in aquaculture for DHA production. |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [41806145]; Science and Technology Program of Guangzhou [201707010174]; Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDA13020203] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [41806145]; Science and Technology Program of Guangzhou [201707010174]; Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDA13020203] |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/18419]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | Chinese Acad Sci, Key Lab Trop Marine Bioresources & Ecol, South China Sea Inst Oceanol, Guangzhou 510301, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Fangfang,Long, Chao,Wei, Zhangliang,et al. Optimization of medium using response surface methodology to enhance the growth of Effrenium voratum (Symbiodiniaceae, Dinophyceae)[J]. JOURNAL OF PHYCOLOGY. |
| APA | Yang, Fangfang,Long, Chao,Wei, Zhangliang,&Long, Lijuan. |
| MLA | Yang, Fangfang,et al."Optimization of medium using response surface methodology to enhance the growth of Effrenium voratum (Symbiodiniaceae, Dinophyceae)".JOURNAL OF PHYCOLOGY |
入库方式： OAI收割
来源：南海海洋研究所
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