Vitellogenin Receptor (VgR) Mediates Oocyte Maturation and Ovarian Development in the Pacific White Shrimp (Litopenaeus vannamei)
文献类型：期刊论文
| 作者 | Ruan, Yao4; Wong, Nai-Kei; Zhang, Xin4; Zhu, Chunhua2; Wu, Xiaofen4; Ren, Chunhua5; Luo, Peng5; Jiang, Xiao5; Ji, Jiatai7; Wu, Xugan6 |
| 刊名 | FRONTIERS IN PHYSIOLOGY
 |
| 出版日期 | 2020 |
| 卷号 | 11页码:485 |
| 关键词 | vitellogenin receptor crustacean phylogenetic analysis vitellogenesis mRNA expression ovarian development oocyte maturation |
| ISSN号 | 1664-042X |
| DOI | 10.3389/fphys.2020.00485 |
| 英文摘要 | Oocyte maturation and ovarian development are sequentially coordinated events critical to reproduction. In the ovaries of adult oviparous animals such as birds, bony fish, insects, and crustaceans, vitellogenin receptor (VgR) is a plasma membrane receptor that specifically mediates vitellogenin (Vg) transport into oocytes. Accumulation of Vg drives sexual maturation of the female crustaceans by acting as a pivotal regulator of nutritional accumulation within oocytes, a process known as vitellogenesis. However, the mechanisms by which VgR mediates vitellogenesis are still not fully understood. In this study, we first identified a unique VgR (Lv-VgR) and characterized its genomic organization and protein structural domains in Litopenaeus vannamei, a predominant cultured shrimp species worldwide. This newly identified Lv-VgR phylogenetically forms a group with VgRs from other crustacean species within the arthropod cluster. Duplicated LBD/EGFD regions are found exclusively among arthropod VgRs but not in paralogs from vertebrates and nematodes. In terms of expression patterns, Lv-VgR transcripts are specifically expressed in ovaries of female shrimps, which increases progressively during ovarian development, and rapidly declines toward embryonic development. The cellular and subcellular locations were For analyzed by in situ hybridization and immunofluorescence, respectively. The Lv-VgR mRNA was found to be expressed in the oocytes of ovaries, and Lv-VgR protein was found to localize in the cell membrane of maturing oocytes while accumulation of the ligand Vg protein assumed an even cytoplasmic distribution. Silencing of VgR transcript expression by RNAi was effective for stunting ovarian development. This present study has thus provided new insights into the regulatory roles of VgR in crustacean ovarian development. |
| 资助机构 | Science & Technology Project of Guangzhou [201904020009]; Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams [2019KJ149]; Guangdong Province Program [2017B030314052, 2017A030310429, 2016A020210062]; Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences [ISEE2018PY03]; Shanghai talents development fund for young scientists [2018100] ; Science & Technology Project of Guangzhou [201904020009]; Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams [2019KJ149]; Guangdong Province Program [2017B030314052, 2017A030310429, 2016A020210062]; Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences [ISEE2018PY03]; Shanghai talents development fund for young scientists [2018100] |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/18426]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Shanghai Ocean Univ, Key Lab Freshwater Aquat Genet Resources, Minist Agr, Shanghai, Peoples R China 2.Southern Univ Sci & Technol, Hosp 2, Natl Clin Res Ctr Infect Dis, Shenzhen Peoples Hosp 3, Shenzhen, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangdong Prov Key Lab Appl Marine Biol LAMB, South China Sea Inst Oceanol, Guangzhou, Peoples R China 5.Guangdong Ocean Univ, Coll Fisheries, Zhanjiang, Peoples R China 6.Guangdong Haimao Investment Co Ltd, Zhanjiang, Peoples R China 7.Chinese Acad Sci, Inst South China Sea Ecol & Environm Engn ISEE, Guangzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ruan, Yao,Wong, Nai-Kei,Zhang, Xin,et al. Vitellogenin Receptor (VgR) Mediates Oocyte Maturation and Ovarian Development in the Pacific White Shrimp (Litopenaeus vannamei)[J]. FRONTIERS IN PHYSIOLOGY,2020,11:485. |
| APA | Ruan, Yao.,Wong, Nai-Kei.,Zhang, Xin.,Zhu, Chunhua.,Wu, Xiaofen.,...&Chen, Ting.(2020).Vitellogenin Receptor (VgR) Mediates Oocyte Maturation and Ovarian Development in the Pacific White Shrimp (Litopenaeus vannamei).FRONTIERS IN PHYSIOLOGY,11,485. |
| MLA | Ruan, Yao,et al."Vitellogenin Receptor (VgR) Mediates Oocyte Maturation and Ovarian Development in the Pacific White Shrimp (Litopenaeus vannamei)".FRONTIERS IN PHYSIOLOGY 11(2020):485. |
入库方式： OAI收割
来源：南海海洋研究所
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