Mechanistic molecular responses of the giant clam Tridacna crocea to Vibrio coralliilyticus challenge
文献类型：期刊论文
| 作者 | Xu, Duo1; Zhao, Zehui1; Zhou, Zihua1; Lin, Yue1; Zhang, Xiangyu1; Zhang, Yang2,3; Zhang, Yuehuan2,3; Li, Jun2,3; Mao, Fan2,3; Xiao, Shu2,3 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2020 |
| 卷号 | 15期号:4页码:e0231399 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0231399 |
| 英文摘要 | Vibrio coralliilyticus is a pathogen of coral and mollusk, contributing to dramatic losses worldwide. In our study, we found that V. coralliilyticus challenge could directly affect adult Tridacna crocea survival; there were dead individuals appearing at 6 h post infection, and there were 45.56% and 56.78% mortality rates in challenged groups after 36 h of infection. The apoptosis rate of hemocytes was significantly increased by 1.8-fold at 6 h after V. coralliilyticus injection. To shed light on the mechanistic molecular responses of T. crocea to V. coralliilyticus infection, we used transcriptome sequencing analysis and other relevant techniques to analyze T. crocea hemocytes at 0 h, 6 h, 12 h and 24 h after V. coralliilyticus challenge. Our results revealed that the total numbers of unigenes and DEGs were 195651 and 3446, respectively. Additional details were found by KEGG pathway enrichment analysis, where DEGs were significantly enriched in immune-related signaling pathways, such as the TLR signaling pathway, and some were associated with signaling related to apoptosis. Quantitative validation results illustrated that with exposure to V. coralliilyticus, the expression of TLR pathway members, TLR, MyD88, IRAK4, TRAF6, and I.B-a, were significantly upregulated (by 22.9-, 9.6-, 4.0-, 3.6-, and 3.9-fold, respectively) at 6 h. The cytokine-related gene IL-17 exhibited an increase of 6.3-fold and 10.5-fold at 3 h and 6 h, respectively. The apoptosis-related gene IAP1 was dramatically increased by 2.99-fold at 6 h. These results indicate that adult T. crocea could initiate the TLR pathway to resist V. coralliilyticus, which promotes the release of inflammatory factors such as IL-17 and leads to the activation of a series of outcomes, such as apoptosis. The response mechanism is related to the T. crocea immunoreaction stimulated by V. coralliilyticus, providing a theoretical basis for understanding T. crocea immune response mechanisms. |
| 资助机构 | Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDA13020403/XDA13020202]; National Key R&D Program of China [2018YFC1406505]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31572661]; Science and Technology Planning Project of Guangzhou, China [2017B030314052, 201707010177]; Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) [GML2019ZD0407]; Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences [ISEE2018PY01]; National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31702340] ; Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDA13020403/XDA13020202]; National Key R&D Program of China [2018YFC1406505]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31572661]; Science and Technology Planning Project of Guangzhou, China [2017B030314052, 201707010177]; Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) [GML2019ZD0407]; Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences [ISEE2018PY01]; National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31702340] |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/18454]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Southern Marine Sci & Engn Guangdong Lab, Guangzhou, Peoples R China 4.Chinese Acad Sci, Innovat Acad South China Sea Ecol & Environm Engn, Guangzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Duo,Zhao, Zehui,Zhou, Zihua,et al. Mechanistic molecular responses of the giant clam Tridacna crocea to Vibrio coralliilyticus challenge[J]. PLOS ONE,2020,15(4):e0231399. |
| APA | Xu, Duo.,Zhao, Zehui.,Zhou, Zihua.,Lin, Yue.,Zhang, Xiangyu.,...&Yu, Ziniu.(2020).Mechanistic molecular responses of the giant clam Tridacna crocea to Vibrio coralliilyticus challenge.PLOS ONE,15(4),e0231399. |
| MLA | Xu, Duo,et al."Mechanistic molecular responses of the giant clam Tridacna crocea to Vibrio coralliilyticus challenge".PLOS ONE 15.4(2020):e0231399. |
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来源：南海海洋研究所
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