Prophage encoding toxin/antitoxin system PfiT/PfiA inhibits Pf4 production in Pseudomonas aeruginosa
文献类型：期刊论文
| 作者 | Li, Yangmei2,3; Liu, Xiaoxiao3; Tang, Kaihao3; Wang, Weiquan2,3; Guo, Yunxue3; Wang, Xiaoxue2,3 |
| 刊名 | MICROBIAL BIOTECHNOLOGY
 |
| 出版日期 | 2020 |
| 卷号 | 13期号:4页码:1132 |
| ISSN号 | 1751-7915 |
| DOI | 10.1111/1751-7915.13570 |
| 英文摘要 | Pf prophages are ssDNA filamentous prophages that are prevalent among various Pseudomonas aeruginosa strains. The genomes of Pf prophages contain not only core genes encoding functions involved in phage replication, structure and assembly but also accessory genes. By studying the accessory genes in the Pf4 prophage in P. aeruginosa PAO1, we provided experimental evidence to demonstrate that PA0729 and the upstream ORF Rorf0727 near the right attachment site of Pf4 form a type II toxin/antitoxin (TA) pair. Importantly, we found that the deletion of the toxin gene PA0729 greatly increased Pf4 phage production. We thus suggest the toxin PA0729 be named PfiT for Pf4 inhibition toxin and Rorf0727 be named PfiA for PfiT antitoxin. The PfiT toxin directly binds to PfiA and functions as a corepressor of PfiA for the TA operon. The PfiAT complex exhibited autoregulation by binding to a palindrome (5 '-AATTCN(5)GTTAA-3 ') overlapping the -35 region of the TA operon. The deletion of pfiT disrupted TA autoregulation and activated pfiA expression. Additionally, the deletion of pfiT also activated the expression of the replication initiation factor gene PA0727. Moreover, the Pf4 phage released from the pfiT deletion mutant overcame the immunity provided by the phage repressor Pf4r. Therefore, this study reveals that the TA systems in Pf prophages can regulate phage production and phage immunity, providing new insights into the function of TAs in mobile genetic elements. |
| 资助机构 | National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31625001, 91951203, 31970037]; National Key R&D Program of China [2018YFC1406500]; National Science Foundation of Guangdong ProvinceNational Natural Science Foundation of Guangdong Province [2017A030313125]; Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDA13020301]; 1000-Youth Elite Program ( Recruitment Program of Global Experts in China) ; National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31625001, 91951203, 31970037]; National Key R&D Program of China [2018YFC1406500]; National Science Foundation of Guangdong ProvinceNational Natural Science Foundation of Guangdong Province [2017A030313125]; Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDA13020301]; 1000-Youth Elite Program ( Recruitment Program of Global Experts in China) |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/18457]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Innovat Acad South China Sea Ecol & Environm Engn, Guangzhou 510301, Peoples R China 3.Chinese Acad Sci, RNAM Ctr Marine Microbiol, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yangmei,Liu, Xiaoxiao,Tang, Kaihao,et al. Prophage encoding toxin/antitoxin system PfiT/PfiA inhibits Pf4 production in Pseudomonas aeruginosa[J]. MICROBIAL BIOTECHNOLOGY,2020,13(4):1132, 1144. |
| APA | Li, Yangmei,Liu, Xiaoxiao,Tang, Kaihao,Wang, Weiquan,Guo, Yunxue,&Wang, Xiaoxue.(2020).Prophage encoding toxin/antitoxin system PfiT/PfiA inhibits Pf4 production in Pseudomonas aeruginosa.MICROBIAL BIOTECHNOLOGY,13(4),1132. |
| MLA | Li, Yangmei,et al."Prophage encoding toxin/antitoxin system PfiT/PfiA inhibits Pf4 production in Pseudomonas aeruginosa".MICROBIAL BIOTECHNOLOGY 13.4(2020):1132. |
入库方式： OAI收割
来源：南海海洋研究所
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