Characterization of Regulatory and Transporter Genes in the Biosynthesis of Anti-Tuberculosis Ilamycins and Production in a Heterologous Host
文献类型：期刊论文
| 作者 | He, Jianqiao3; Wei, Xin3; Yang, Zhijie3; Li, Yan; Ju, Jianhua1,3; Ma, Junying1 |
| 刊名 | MARINE DRUGS
 |
| 出版日期 | 2020 |
| 卷号 | 18期号:4页码:216 |
| 关键词 | marine-derived Streptomyces ilamycins regulator ABC-transporter heterologous expression |
| DOI | 10.3390/md18040216 |
| 英文摘要 | Ilamycins are cyclopeptides with novel structures that have been isolated from different Actinomycetes. They showed strong anti-tuberculosis activity and could serve as important anti-tuberculosis drug leads. The functions of the pre-tailoring and the post-tailoring genes in the biosynthesis of ilamycins have been elucidated, but the functions of the regulatory and transporter genes remain elusive. We reported herein the functions of four genes in ilamycin biosynthetic gene cluster (ila BGC) including two regulatory genes (ilaA and ilaB) and two transporter genes (ilaJ and ilaK) and the heterologous expression of ila BGC. The IlaA and IlaB were unambiguously shown to be negative and positive regulator of ilamycins biosynthesis, respectively. Consistent with these roles, inactivation of ilaA and ilaB (independent of each other) was shown to enhance and abolish the production of ilamycins, respectively. Total yields of ilamycins were enhanced 3.0-fold and 1.9-fold by inactivation of ilaA and overexpression of ilaB compared to those of in the Streptomyces atratus SCSIO ZH16, respectively. In addition, the ila BGC was successfully expressed in Streptomyces coelicolor M1152, which indicated that all biosynthetic elements for the construction of ilamycins were included in the PAC7A6. These results not only lay a foundation for further exploration of ilamycins, but also provide the genetic elements for synthetic biology. |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31870046, U1706206, 20872152]; Special Funds for Economic Development of Marine Economy of Guangdong Province [GMDE-2018C003]; Key Special project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) [GML2019ZD0406]; National Natural Science Foundation of Guangdong ProvinceNational Natural Science Foundation of Guangdong Province [2018A0303130005] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31870046, U1706206, 20872152]; Special Funds for Economic Development of Marine Economy of Guangdong Province [GMDE-2018C003]; Key Special project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) [GML2019ZD0406]; National Natural Science Foundation of Guangdong ProvinceNational Natural Science Foundation of Guangdong Province [2018A0303130005] |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/18460]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Key Special Project Introduced Talents Team South, Guangzhou 510301, Peoples R China 3.Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med, RNAM Ctr Marine Microbiol,South China Sea Inst Oc, Guangzhou 510301, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Jianqiao,Wei, Xin,Yang, Zhijie,et al. Characterization of Regulatory and Transporter Genes in the Biosynthesis of Anti-Tuberculosis Ilamycins and Production in a Heterologous Host[J]. MARINE DRUGS,2020,18(4):216. |
| APA | He, Jianqiao,Wei, Xin,Yang, Zhijie,Li, Yan,Ju, Jianhua,&Ma, Junying.(2020).Characterization of Regulatory and Transporter Genes in the Biosynthesis of Anti-Tuberculosis Ilamycins and Production in a Heterologous Host.MARINE DRUGS,18(4),216. |
| MLA | He, Jianqiao,et al."Characterization of Regulatory and Transporter Genes in the Biosynthesis of Anti-Tuberculosis Ilamycins and Production in a Heterologous Host".MARINE DRUGS 18.4(2020):216. |
入库方式： OAI收割
来源：南海海洋研究所
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