A rapid and reliable method for identifying genetic sex in obscure pufferfish (Takifugu obscures)
文献类型：期刊论文
| 作者 | Gao, Fan-Xiang; Shi, Yan; Duan, Wen; Lu, Wei-Jia; Huang, Wen2; Zhang, Xiao-Juan; Zhao, Zhe; Zhou, Li; Gui, Jian-Fang |
| 刊名 | AQUACULTURE
 |
| 出版日期 | 2020 |
| 卷号 | 519页码:734749 |
| 关键词 | Obscure pufferfish Takifugu obscurus Amhr2 Sex-specific marker Genetic sex identification Sex determination |
| ISSN号 | 0044-8486 |
| DOI | 10.1016/j.aquaculture.2019.734749 |
| 英文摘要 | Obscure pufferfish (Takifugu obscurus) is an important aquaculture species in China, and sex control breeding is extremely attractive in this species because the testes of male pufferfish are more popular and expensive. However, the sex-specific marker remains unknown and reliable genetic sex identification method has not been established, which has hampered the development of monosexual aquaculture. In this study, the sex-linked amhr2 SNP site of obscure pufferfish was firstly identified by sequencing and high-resolution melting (HRM) analysis. The males exhibit heterozygous genotype (C/G), while females have homozygous genotype (C/C), which is identical to its closely-related species (T. rubripes, T. poecilonotus and T. pardalis). Then, two pairs of male-specific primers were designed based on the sex-specific SNP site and a high-throughput sexing method was subsequently developed with benefits including high specificity and superb sensitivity. Particularly, this method has greatly reduced analysis time and costs compared to the previously established HRM analysis. These results will be beneficial to develop the sex control breeding in obscure pufferfish. |
| 资助机构 | National Key Research and Development Program of China, China [2018YFD0900200]; State Key Laboratory of Freshwater Ecology and Biotechnology, China [2019FB06]; Fundamental Research Funds for the Central Universities, ChinaFundamental Research Funds for the Central Universities [2018B07114]; Key Laboratory of Marine Bio-resources Sustainable Utilization, China [2019011008]; Guangdong Key Laboratory of Applied Marine Biology, China [2019011008]; Guangdong Key Laboratory of Marine Materia Medica, China [2019011008] ; National Key Research and Development Program of China, China [2018YFD0900200]; State Key Laboratory of Freshwater Ecology and Biotechnology, China [2019FB06]; Fundamental Research Funds for the Central Universities, ChinaFundamental Research Funds for the Central Universities [2018B07114]; Key Laboratory of Marine Bio-resources Sustainable Utilization, China [2019011008]; Guangdong Key Laboratory of Applied Marine Biology, China [2019011008]; Guangdong Key Laboratory of Marine Materia Medica, China [2019011008] |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/18470]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Hohai Univ, Coll Oceanog, Inst Marine Biol, Nanjing 210098, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 3.Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Key Lab Appl Marine Biol Guangdong Prov, Guangzhou 510301, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Fan-Xiang,Shi, Yan,Duan, Wen,et al. A rapid and reliable method for identifying genetic sex in obscure pufferfish (Takifugu obscures)[J]. AQUACULTURE,2020,519:734749. |
| APA | Gao, Fan-Xiang.,Shi, Yan.,Duan, Wen.,Lu, Wei-Jia.,Huang, Wen.,...&Gui, Jian-Fang.(2020).A rapid and reliable method for identifying genetic sex in obscure pufferfish (Takifugu obscures).AQUACULTURE,519,734749. |
| MLA | Gao, Fan-Xiang,et al."A rapid and reliable method for identifying genetic sex in obscure pufferfish (Takifugu obscures)".AQUACULTURE 519(2020):734749. |
入库方式： OAI收割
来源：南海海洋研究所
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