Phagocyte Transcriptomic Analysis Reveals Focal Adhesion Kinase (FAK) and Heparan Sulfate Proteoglycans (HSPGs) as Major Regulators in Anti-bacterial Defense of Crassostrea hongkongensis
文献类型：期刊论文
| 作者 | Lin, Yue1; Mao, Fan2,3; Wong, Nai-Kei; Zhang, Xiangyu1; Liu, Kunna1; Huang, Minwei2,3; Ma, Haitao2,3; Xiang, Zhiming2,3; Li, Jun2,3; Xiao, Shu2,3 |
| 刊名 | FRONTIERS IN IMMUNOLOGY
 |
| 出版日期 | 2020 |
| 卷号 | 11页码:416 |
| 关键词 | phagocytes heparan sulfate proteoglycans (HSPGs) focal adhesion kinase (FAK) transcriptome Crassostrea hongkongensis |
| ISSN号 | 1664-3224 |
| DOI | 10.3389/fimmu.2020.00416 |
| 英文摘要 | Invertebrates generally lack adaptive immunity and compensate for this with highly efficient innate immune machineries such as phagocytosis by hemocytes to eradicate invading pathogens. However, how extrinsically cued hemocytes marshal internal signals to accomplish phagocytosis is not yet fully understood. To this end, we established a facile magnetic cell sorting method to enrich professional phagocytes from hemocytes of the Hong Kong oyster (Crassostrea hongkongensis), an ecologically and commercially valuable marine invertebrate. Transcriptomic analysis on presorted cells shows that phagocytes maintain a remarkable array of differentially expressed genes that distinguish them from non-phagocytes, including 352 significantly upregulated genes and 479 downregulated genes. Pathway annotations reveal that focal adhesion and extracellular matrix-receptor interactions were the most conspicuously enriched pathways in phagocytes. Phagocytosis rate dramatically declined in the presence of an FAK inhibitor, confirming importance of the focal adhesion pathway in regulating phagocytosis. In addition, we also found that heparan sulfate proteoglycan (HSPG) families were lineage-specifically expanded in C. hongkongensis and abundantly expressed in phagocytes. Efficiency of phagocytosis and hemocytes aggregation was markedly reduced upon blockage of endogenous synthesis of HSPGs, thus implicating these proteins as key surface receptors in pathogen recognition and initiation of phagocytosis. |
| 资助机构 | National Key R&D Program of China [2018YFC1406505]; National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31572640, 31572661]; Science and Technology Program of Guangzhou, China [201804020073]; Guangdong Special Support Program of Youth Scientific and Technological Innovation [2015TQ01N139]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [201806010003]; Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences [ISEE2018PY01, ISEE2018PY03, ISEE2018ZD01]; Science and Technology Planning Project of Guangdong Province, China [2017B030314052, 201707010177] ; National Key R&D Program of China [2018YFC1406505]; National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31572640, 31572661]; Science and Technology Program of Guangzhou, China [201804020073]; Guangdong Special Support Program of Youth Scientific and Technological Innovation [2015TQ01N139]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [201806010003]; Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences [ISEE2018PY01, ISEE2018PY03, ISEE2018ZD01]; Science and Technology Planning Project of Guangdong Province, China [2017B030314052, 201707010177] |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/18474]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Innovat Acad South China Sea Ecol & Environm Engn, Guangzhou, Peoples R China 4.Southern Marine Sci & Engn Guangdong Lab, Guangzhou, Peoples R China 5.Southern Univ Sci & Technol Shenzhen, Hosp 2, Shenzhen Peoples Hosp 3, Natl Clin Res Ctr Infect Dis, Shenzhen, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, Yue,Mao, Fan,Wong, Nai-Kei,et al. Phagocyte Transcriptomic Analysis Reveals Focal Adhesion Kinase (FAK) and Heparan Sulfate Proteoglycans (HSPGs) as Major Regulators in Anti-bacterial Defense of Crassostrea hongkongensis[J]. FRONTIERS IN IMMUNOLOGY,2020,11:416. |
| APA | Lin, Yue.,Mao, Fan.,Wong, Nai-Kei.,Zhang, Xiangyu.,Liu, Kunna.,...&Yu, Ziniu.(2020).Phagocyte Transcriptomic Analysis Reveals Focal Adhesion Kinase (FAK) and Heparan Sulfate Proteoglycans (HSPGs) as Major Regulators in Anti-bacterial Defense of Crassostrea hongkongensis.FRONTIERS IN IMMUNOLOGY,11,416. |
| MLA | Lin, Yue,et al."Phagocyte Transcriptomic Analysis Reveals Focal Adhesion Kinase (FAK) and Heparan Sulfate Proteoglycans (HSPGs) as Major Regulators in Anti-bacterial Defense of Crassostrea hongkongensis".FRONTIERS IN IMMUNOLOGY 11(2020):416. |
入库方式： OAI收割
来源：南海海洋研究所
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