Anti-HSV-1 activity of Aspergillipeptide D, a cyclic pentapepetide isolated from fungus Aspergillus sp. SCSIO 41501
文献类型：期刊论文
| 作者 | Wang, Zhaoyang; Jia, Jiaoyan; Wang, Lu; Li, Feng; Wang, Yiliang; Jiang, Yuzhou; Song, Xiaowei; Qin, Shurong; Zheng, Kai1; Ye, Ju2 |
| 刊名 | VIROLOGY JOURNAL
 |
| 出版日期 | 2020 |
| 卷号 | 17期号:1页码:41 |
| 关键词 | HSV-1 Aspergillipeptide D Marine peptide Glycoprotein B |
| DOI | 10.1186/s12985-020-01315-z |
| 英文摘要 | Background Herpes simplex virus 1, an enveloped DNA virus belonging to the Herpesviridae family, spreads to neurons and causes pathological changes in the central nervous system. The purpose of this study was to investigate the potency and mechanism of antiviral activity of Aspergillipeptide D, a cyclic pentapeptide isolated from a culture broth of marine gorgonian-derived fungus Aspergillus sp. SCSIO 41501, At present, there are many studies on the anti-tumor, anti-clotting, anti-oxidant and immunoinflammatory effects of Aspergillipeptide D, but little research has been done on the anti-HSV-1 activity of Aspergillipeptide D. Methods The anti-HSV-1 activity of Aspergillipeptide D was evaluated by plaque reduction assay. The mechanism of action against HSV-1 was determined from the effective stage. Then we assayed the viral DNA replication, viral RNA synthesis and protein expression, respectively. We also identified the proteins that interact with gB by mass spectrometry, and assayed the effect of Aspergillipeptide D on the interaction between the virus gB protein and cell proteins. Results Plaque reduction experiments showed that Aspergillipeptide D did not affect HSV-1 early infection events, including viral inactivation, attachment and penetration. Interestingly, Aspergillipeptide D dramatically reduced both the gene and protein levels of viral late protein gB, and suppressed its location in the endoplasmic reticulum and Golgi apparatus. In contrast, overexpression of gB restored viral production. Finally, proteomic analysis revealed that the numbers of cellular proteins that interacted with gB protein was largely decreased by Aspergillipeptide D. These results suggested that Aspergillipeptide D inhibited gB function to affect HSV-1 intercellular spread. Conclusions Our results indicated that Aspergillipeptide D might be a potential candidate for HSV-1 therapy, especially for ACV-resistant strains. |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81573471, 81673326]; National Key Research and Development Program of China; International Cooperation Program of Guangdong Province of China [2015A050502028]; Industry-university Collaborative Innovative Major Projects of Guangzhou [201504291048224]; Science and Technology Planning Project of Guangdong Province, China [2016A040402033]; Key Laboratory of Bioengineering Drugs of Guangdong Province [2014B030301050-5]; Basic Research Project of Qinghai Province of China [2016-ZJ-712]; Guangdong Natural Science FoundationNational Natural Science Foundation of Guangdong Province [2019A1515010046] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81573471, 81673326]; National Key Research and Development Program of China; International Cooperation Program of Guangdong Province of China [2015A050502028]; Industry-university Collaborative Innovative Major Projects of Guangzhou [201504291048224]; Science and Technology Planning Project of Guangdong Province, China [2016A040402033]; Key Laboratory of Bioengineering Drugs of Guangdong Province [2014B030301050-5]; Basic Research Project of Qinghai Province of China [2016-ZJ-712]; Guangdong Natural Science FoundationNational Natural Science Foundation of Guangdong Province [2019A1515010046] |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/18475]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Jinan Univ, Guangzhou Jinan Biomed Res & Dev Ctr, Natl Engn Res Ctr Genet Med, Guangzhou, Guangdong, Peoples R China 2.Shenzhen Univ, Sch Pharmaceut Sci, Hlth Sci Ctr, Shenzhen, Peoples R China 3.Qinghai Univ Nationalities, Key Lab Plant Chem Qinghai Tibet Plateau, Xining 810007, Qinghai, Peoples R China 4.Chinese Acad Sci, CAS Key Lab Trop Marine Bio Resources & Ecol, South China Sea Inst Oceanol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Zhaoyang,Jia, Jiaoyan,Wang, Lu,et al. Anti-HSV-1 activity of Aspergillipeptide D, a cyclic pentapepetide isolated from fungus Aspergillus sp. SCSIO 41501[J]. VIROLOGY JOURNAL,2020,17(1):41. |
| APA | Wang, Zhaoyang.,Jia, Jiaoyan.,Wang, Lu.,Li, Feng.,Wang, Yiliang.,...&Qi, Shuhua.(2020).Anti-HSV-1 activity of Aspergillipeptide D, a cyclic pentapepetide isolated from fungus Aspergillus sp. SCSIO 41501.VIROLOGY JOURNAL,17(1),41. |
| MLA | Wang, Zhaoyang,et al."Anti-HSV-1 activity of Aspergillipeptide D, a cyclic pentapepetide isolated from fungus Aspergillus sp. SCSIO 41501".VIROLOGY JOURNAL 17.1(2020):41. |
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来源：南海海洋研究所
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