Characterization and functional analysis of a caspase 3 gene: Evidence that ChCas 3 participates in the regulation of apoptosis in Crassostrea hongkongensis
文献类型：期刊论文
| 作者 | Qin, Yanping1,3; Zhang, Yuehuan3; Li, Xingyou1,3; Noor, Zohaib1,3; Li, Jun3; Zhou, Zihua1,3; Xu, Duo1,3; Zhao, Zehui1,3; Xiang, Zhiming3; Yu, Ziniu3 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2020 |
| 卷号 | 98页码:122 |
| ISSN号 | 1050-4648 |
| 关键词 | Crassostrea hongkongensis Caspase 3 Apoptosis Reporter genes dsRNA |
| DOI | 10.1016/j.fsi.2020.01.007 |
| 英文摘要 | Caspase 3 plays an important role in apoptotic pathways and contributes to maintaining the homeostasis of the immune system in organisms. The structure, functions, and characteristics of caspase 3 have been extensively investigated in many species, but the research is scarce when it comes to bivalves, particularly oysters. In this study, we identified and cloned a previously unknown caspase 3 gene, named ChCas 3, in C. hongkongensis. The full-length cDNA of ChCas 3 was 1562 bp and included a 175 bp 5'-untranslated region (UTR), a 141 bp 3'-UTR and a 1245 bp open reading frame (ORF) that encoded a polypeptide of 415 amino adds. Similar to caspase 3 in other species, ChCas 3 has a pro-domain, a conserved cysteine active site, a large p20 subunit and a small p10 subunit. Our findings demonstrated the expression of ChCas 3 in all the eight tissues via tissue-specific expression assays with the highest expression in haemocytes. ChCas 3 was confirmed to be expressed throughout the larval development stages, and fluorescence from pEGFP-N1-ChCas 3 was found to be distributed throughout the entire HEK293T cell. In addition, the relative expression of ChCas 3 significantly enhanced in hemocytes post bacterial stimulation, and overexpression of ChCas 3 led to upregulation of the transcriptional activity of NF-kappa B and p53 reporter genes in HEK293T cells, which indicated that it was involved in innate immune responses. Finally, the apoptosis rate of the haemocytes declined considerably compared with that of the control group after the expression of ChCas 3 was successfully silenced by dsRNA, corroborating its sentinel role in apoptosis. This study provides comprehensive underpinning evidences, affirming caspase 3 crucial role against bacterial infection and apoptosis in C. hongkongensis. |
| 资助机构 | Science and Technology Planning Project of Guangzhou, China [201707010177]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31572661, 31572640, 31702340, 2015A030]; Chinese Ministry of Science and Technology through the National Key Research and Development Program of China [2018YFD0901400]; Innovation Academy of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences [ISEE2018PY01, ISEE2018ZD02, ISEE2018PY03]; Science and Technology Planning Project of Guangdong Province, China [2017B030314052]; China Agricultural Research System [CARS-49] ; Science and Technology Planning Project of Guangzhou, China [201707010177]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31572661, 31572640, 31702340, 2015A030]; Chinese Ministry of Science and Technology through the National Key Research and Development Program of China [2018YFD0901400]; Innovation Academy of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences [ISEE2018PY01, ISEE2018ZD02, ISEE2018PY03]; Science and Technology Planning Project of Guangdong Province, China [2017B030314052]; China Agricultural Research System [CARS-49] |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/18488]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Peoples R China 2.Chinese Acad Sci, Innovat Acad South China Sea Ecol & Environm Engn, Guangzhou 510301, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qin, Yanping,Zhang, Yuehuan,Li, Xingyou,et al. Characterization and functional analysis of a caspase 3 gene: Evidence that ChCas 3 participates in the regulation of apoptosis in Crassostrea hongkongensis[J]. FISH & SHELLFISH IMMUNOLOGY,2020,98:122, 129. |
| APA | Qin, Yanping.,Zhang, Yuehuan.,Li, Xingyou.,Noor, Zohaib.,Li, Jun.,...&Yu, Ziniu.(2020).Characterization and functional analysis of a caspase 3 gene: Evidence that ChCas 3 participates in the regulation of apoptosis in Crassostrea hongkongensis.FISH & SHELLFISH IMMUNOLOGY,98,122. |
| MLA | Qin, Yanping,et al."Characterization and functional analysis of a caspase 3 gene: Evidence that ChCas 3 participates in the regulation of apoptosis in Crassostrea hongkongensis".FISH & SHELLFISH IMMUNOLOGY 98(2020):122. |
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来源：南海海洋研究所
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