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The Isolation of Pyrroloformamide Congeners and Characterization of Their Biosynthetic Gene Cluster

文献类型：期刊论文

作者Zhou, Wenqing; Liang, Haoyu; Qin, Xiangjing5; Cao, Danfeng; Zhu, Xiangcheng3; Ju, Jianhua5; Shen, Ben1,6,7; Duan, Yanwen3,4; Huang, Yong4
刊名JOURNAL OF NATURAL PRODUCTS
出版日期2020
卷号83期号:2页码:202
ISSN号0163-3864
DOI10.1021/acs.jnatprod.9b00321
英文摘要Dithiolopyrrolones are microbial natural products containing a disulfide or thiosulfonate bridge embedded in a unique bicyclic structure. By interfering with zinc ion homeostasis in living cells, they show strong antibacterial activity against a variety of bacterial pathogens, as well as potent cytotoxicity against human cancer cells. In the current study, two new dithiolopyrrolones, pyrroloformamide C (3) and pyrroloformamide D (4), were isolated from Streptomyces sp. CB02980, together with the known pyrroloformamides 1 and 2. The biosynthetic gene cluster for pyrroloformamides was identified from Streptomyces sp. CB02980, which shared high sequence similarity with those of dithiolopyrrolones, including holomycin and thiolutin. Gene replacement of pyfE, which encodes a nonribosomal peptide synthetase (NRPS), abolished the production of 1-4. Overexpression of pyfN, a type II thioesterase gene, increased the production of 1 and 2. Genome neighborhood network analysis of the characterized and orphan gene clusters of dithiolopyrrolones revealed a unified mechanism for their biosynthesis, involving an iterative-acting NRPS and a set of conserved tailoring enzymes for the bicyclic core formation.
资助机构NSFCNational Natural Science Foundation of China (NSFC) [81473124, 81530092]; Chinese Ministry of Education 111 Project [B0803420] ; NSFCNational Natural Science Foundation of China (NSFC) [81473124, 81530092]; Chinese Ministry of Education 111 Project [B0803420]
源URL[http://ir.scsio.ac.cn/handle/344004/18502]  
专题南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
作者单位1.Scripps Res, Dept Mol Med, Jupiter, FL 33458 USA
2.Scripps Res, Dept Nat Prod Lib Initiat, Jupiter, FL 33458 USA
3.Cent South Univ, Xiangya Int Acad Translat Med, Changsha 410013, Peoples R China
4.Hunan Engn Res Ctr Combinatorial Biosynth & Nat P, Changsha 410011, Hunan, Peoples R China
5.Natl Engn Res Ctr Combinatorial Biosynth Drug Dis, Changsha 410011, Hunan, Peoples R China
6.Chinese Acad Sci, South China Sea Inst Oceanol, RNAM Ctr Marine Microbiol, Guangdong Key Lab Marine Mat,CAS Key Lab Trop Mar, Guangzhou 510301, Peoples R China
7.Scripps Res, Dept Chem, Jupiter, FL 33458 USA
推荐引用方式
GB/T 7714		 Zhou, Wenqing,Liang, Haoyu,Qin, Xiangjing,et al. The Isolation of Pyrroloformamide Congeners and Characterization of Their Biosynthetic Gene Cluster[J]. JOURNAL OF NATURAL PRODUCTS,2020,83(2):202, 209.
APA Zhou, Wenqing.,Liang, Haoyu.,Qin, Xiangjing.,Cao, Danfeng.,Zhu, Xiangcheng.,...&Huang, Yong.(2020).The Isolation of Pyrroloformamide Congeners and Characterization of Their Biosynthetic Gene Cluster.JOURNAL OF NATURAL PRODUCTS,83(2),202.
MLA Zhou, Wenqing,et al."The Isolation of Pyrroloformamide Congeners and Characterization of Their Biosynthetic Gene Cluster".JOURNAL OF NATURAL PRODUCTS 83.2(2020):202.

入库方式： OAI收割

来源：南海海洋研究所

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