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TRAF6 suppresses the apoptosis of hemocytes by activating pellino in Crassostrea hongkongensis

文献类型：期刊论文

作者Lin, Yue1; Mao, Fan3; Zhang, Xiangyu1; Xu, Duo1; He, Zhiying1; Li, Jun3; Xiang, Zhiming3; Zhang, Yang3; Yu, Ziniu3
刊名DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
出版日期2020
卷号103页码:103501
关键词Crassostrea hongkongensis ChTRAF6 ChPellino Apoptosis Innate immunity
ISSN号0145-305X
DOI10.1016/j.dci.2019.103501
英文摘要Tumor necrosis factor receptor-associated factor 6 (TRAF6), an E3 ubiquitin ligase, participates in both innate and adaptive immunity and regulates the apoptotic process. In this study, we observed that an ortholog of TRAF6 could inhibit the activity of p53 and suppress the apoptotic process in the Hong Kong oyster, Crassostrea hongkongensis. To investigate the possible molecular mechanism of the ChTRAF6-induced antiapoptotic effect, a GST pull-down screening assay was conducted, and ChPellino was found to physically interact with ChTRAF6. In addition, the interaction between them was confirmed by Co-immunoprecipitation. Furthermore, western blotting revealed that the phosphorylation level of ChPellino was decreased after the RNAi of ChTRAF6, demonstrating that ChTRAF6 may be an upstream regulator of Pellino activation. Furthermore, the apoptosis level of hemocytes increased after ChPellino knockdown, and ChPellino overexpression suppressed ChTRAF6-dependent p53 activation. Taken together, these results indicate that ChPellino plays a critical role in suppressing ChTRAF6-dependent anti-apoptosis in the hemocytes of Crassostrea hongkongensis.
资助机构Guangdong Natural Science Funds for Distinguished Young Scholars [2015A030306003]; National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31572640, 31572661]; Guangdong Special Support Program of Youth Scientific and Technological Innovation [2015TQ01N139]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [201806010003]; Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences [ISEE2018PY03]; Science and Technology Planning Project of Guangzhou, China [2017B030314052, 201707010177] ; Guangdong Natural Science Funds for Distinguished Young Scholars [2015A030306003]; National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31572640, 31572661]; Guangdong Special Support Program of Youth Scientific and Technological Innovation [2015TQ01N139]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [201806010003]; Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences [ISEE2018PY03]; Science and Technology Planning Project of Guangzhou, China [2017B030314052, 201707010177]
源URL[http://ir.scsio.ac.cn/handle/344004/18505]  
专题南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
作者单位1.Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
2.Chinese Acad Sci, Innovat Acad South China Sea Ecol & Environm Engn, Beijing, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Lin, Yue,Mao, Fan,Zhang, Xiangyu,et al. TRAF6 suppresses the apoptosis of hemocytes by activating pellino in Crassostrea hongkongensis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2020,103:103501.
APA Lin, Yue.,Mao, Fan.,Zhang, Xiangyu.,Xu, Duo.,He, Zhiying.,...&Yu, Ziniu.(2020).TRAF6 suppresses the apoptosis of hemocytes by activating pellino in Crassostrea hongkongensis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,103,103501.
MLA Lin, Yue,et al."TRAF6 suppresses the apoptosis of hemocytes by activating pellino in Crassostrea hongkongensis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 103(2020):103501.

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来源：南海海洋研究所

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