The role of a new insulin-like peptide in the pearl oyster Pinctada fucata martensii
文献类型：期刊论文
| 作者 | Zhang, Hua1; He, Maoxian1 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2020 |
| 卷号 | 10期号:1页码:433 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/s41598-019-57329-3 |
| 英文摘要 | Pinctada fucata martensii, is an economically important marine bivalve species cultured for seawater pearls. At present, we know little about the molecular mechanisms of the insulin signalling pathway in this oyster. Herein, we cloned and analysed an insulin-like peptide (PfILP) and its signalling pathway-related genes. We detected their expression levels in different tissues and developmental stages. Recombinant PfILP protein was produced and found to significantly increase primary mantle cell activity and induce the expression of the proliferating cell nuclear antigen (PCNA) gene. PfILP could also regulate the 293T cell cycle by stimulating the S phase and inhibiting the G1 and G2 phases. Recombinant PfILP protein induced the expression of its signalling pathway-related genes in mantle cells. In vitro co-immunoprecipitation analysis showed that PfILP interacts with PfIRR. PfILP activated expression of the pfIRR protein, and also activated the mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) pathways by stimulating phosphorylation of MAPK and AKT. Further analysis showed that PfILP up-regulated glycogen synthesis-related genes glycogen synthase kinase-3 beta (GSK-3 beta), protein phosphatase 1 (PP1) and glucokinase (GK) at the mRNA level, as well as the expression of the PP1 protein, and phosphorylation of GSK-3 beta. These results confirmed the presence of a conserved insulin-like signalling pathway in pearl oyster that is involved in cell activity, glycogen metabolism, and other physiological processes. |
| 资助机构 | earmarked fund for Modern Agro-industry Technology Research System [CARS-49]; Science and Technology Planning Project of Guangdong Province, China [2014B030301064, 2017B030314052]; Natural Science Foundation of Guangdong Province, ChinaNational Natural Science Foundation of Guangdong Province [2015A030310362] ; earmarked fund for Modern Agro-industry Technology Research System [CARS-49]; Science and Technology Planning Project of Guangdong Province, China [2014B030301064, 2017B030314052]; Natural Science Foundation of Guangdong Province, ChinaNational Natural Science Foundation of Guangdong Province [2015A030310362] |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/18512]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Peoples R China 2.Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Hua,He, Maoxian. The role of a new insulin-like peptide in the pearl oyster Pinctada fucata martensii[J]. SCIENTIFIC REPORTS,2020,10(1):433. |
| APA | Zhang, Hua,&He, Maoxian.(2020).The role of a new insulin-like peptide in the pearl oyster Pinctada fucata martensii.SCIENTIFIC REPORTS,10(1),433. |
| MLA | Zhang, Hua,et al."The role of a new insulin-like peptide in the pearl oyster Pinctada fucata martensii".SCIENTIFIC REPORTS 10.1(2020):433. |
入库方式： OAI收割
来源：南海海洋研究所
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