Colistin Resistance Gene mcr-1 Mediates Cell Permeability and Resistance to Hydrophobic Antibiotics
文献类型：期刊论文
| 作者 | Li, Baiyuan; Yin, Fang2; Zhao, Xuanyu3,4; Guo, Yunxue3; Wang, Weiquan3,4; Wang, Pengxia3; Zhu, Honghui5; Yin, Yeshi; Wang, Xiaoxue3,4 |
| 刊名 | FRONTIERS IN MICROBIOLOGY
 |
| 出版日期 | 2020 |
| 卷号 | 10页码:3015 |
| 关键词 | mcr-1 colistin resistance permeability hydrophobic antibiotics plasmid |
| ISSN号 | 1664-302X |
| DOI | 10.3389/fmicb.2019.03015 |
| 英文摘要 | Colistin is considered the last-resort antibiotic used to treat multidrug resistant bacteria-related infections. However, the discovery of the plasmid-mediated colistin resistance gene, mcr-1, threatens the clinical utility of colistin antibiotics. In this study, the physiological function of MCR-1, which encodes an LPS-modifying enzyme, was investigated in E. coli K-12. Specifically, the impact of mcr-1 on membrane permeability and antibiotic resistance of E. coli was assessed by constructing an mcr-1 deletion mutant and by a complementation study. The removal of the mcr-1 gene from plasmid pHNSHP45 not only led to reduced resistance to colistin but also resulted in a significant change in the membrane permeability of E. coli. Unexpectedly, the removal of the mcr-1 gene increased cell viability under high osmotic stress conditions (e.g., 7.0% NaCl) and led to increased resistance to hydrophobic antibiotics. Increased expression of mcr-1 also resulted in decreased growth rate and changed the cellular morphology of E. coli. Collectively, our results revealed that the spread of mcr-1-carrying plasmids alters other physiological functions in addition to conferring colistin resistance. |
| 资助机构 | National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31290233, 41230962, 41406189, 31470141]; Chinese Academy of SciencesChinese Academy of Sciences [XDA11030402]; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2017M622649]; Science and Technology Innovation Leading Talent Program of Guangdong Province [2015TX01N036]; 1000-Youth Elite Program award ; National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31290233, 41230962, 41406189, 31470141]; Chinese Academy of SciencesChinese Academy of Sciences [XDA11030402]; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2017M622649]; Science and Technology Innovation Leading Talent Program of Guangdong Province [2015TX01N036]; 1000-Youth Elite Program award |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/18516]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Guangdong Inst Microbiol, Guangdong Prov Key Lab Microbial Culture Collect, Guangdong Open Lab Appl Microbiol, Guangdong Microbial Culture Collect Ctr,State Key, Guangzhou, Peoples R China 2.Hunan Univ Sci & Engn, Coll Chem & Bioengn, Key Lab Comprehens Utilizat Advantage Plants Reso, Yongzhou, Peoples R China 3.Sun Yat Sen Univ, Affiliated Hosp 5, Dept Breast & Thyroid Surg, Zhuhai, Peoples R China 4.Chinese Acad Sci, Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med, RNAM Ctr Marine Microbiol,South China Sea Inst Oc, Guangzhou, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Baiyuan,Yin, Fang,Zhao, Xuanyu,et al. Colistin Resistance Gene mcr-1 Mediates Cell Permeability and Resistance to Hydrophobic Antibiotics[J]. FRONTIERS IN MICROBIOLOGY,2020,10:3015. |
| APA | Li, Baiyuan.,Yin, Fang.,Zhao, Xuanyu.,Guo, Yunxue.,Wang, Weiquan.,...&Wang, Xiaoxue.(2020).Colistin Resistance Gene mcr-1 Mediates Cell Permeability and Resistance to Hydrophobic Antibiotics.FRONTIERS IN MICROBIOLOGY,10,3015. |
| MLA | Li, Baiyuan,et al."Colistin Resistance Gene mcr-1 Mediates Cell Permeability and Resistance to Hydrophobic Antibiotics".FRONTIERS IN MICROBIOLOGY 10(2020):3015. |
入库方式： OAI收割
来源：南海海洋研究所
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