Two Distinct C-Type Lysozymes in Goldfish: Molecular Characterization, Antimicrobial Potential, and Transcriptional Regulation in Response to Opposing Effects of Bacteria/Lipopolysaccharide and Dexamethasone/Leptin
文献类型：期刊论文
| 作者 | Chen, Ting3,4; Rao, Yingzhu; Li, Jiaxi5; Ren, Chunhua3,4; Tang, Dongsheng5; Lin, Tiehao6; Ji, Jiatai1,4; Chen, Rong; Yan, Aifen5 |
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2020 |
| 卷号 | 21期号:2页码:501 |
| 关键词 | c-type lysozyme fish antimicrobial activity innate immunity dexamethasone leptin |
| DOI | 10.3390/ijms21020501 |
| 英文摘要 | Lysozymes are key antimicrobial peptides in the host innate immune system that protect against pathogen infection. In this study, the full-length cDNAs of two c-type lysozymes (gfLyz-C1 and gfLyz-C2) were cloned from goldfish (Carassius auratus). The structural domains, three-dimensional structures, and amino acid sequences of gfLyz-C1 and gfLyz-C2 were highly comparable, as the two proteins shared 89.7% sequence identity. The gfLyz-C1 and gfLyz-C2 recombinant proteins were generated in the insoluble fractions of an Escherichia coli system. Based on the results of lysoplate and turbidimetric assays, gfLyz-C1 and gfLyz-C2 showed broad-spectrum antimicrobial properties with high levels of activity against Micrococcus lysodeikticus, Vibrio parahemolyticus, and Edwardsiella tarda, and relatively low activity against E. coli. Both gfLyz-C1 and gfLyz-C2 mRNAs were mainly expressed in the trunk kidney and head kidney, and gfLyz-C1 was expressed at much higher levels than gfLyz-C2 in the corresponding tissues. The expression of the gfLyz-C1 and gfLyz-C2 transcripts in the trunk kidney and head kidney was induced in these tissues by challenge with heat-inactivated E. coli and lipopolysaccharides (LPS), and the transcriptional responses of gfLyz-C1 were more intense. In goldfish primary trunk kidney cells, the levels of the gfLyz-C1 and gfLyz-C2 transcripts were upregulated by heat-inactivated E. coli, V. parahemolyticus, and E. tarda, as well as LPS, and downregulated by treatment with dexamethasone and leptins. Overall, this study may provide new insights that will improve our understanding of the roles of c-type lysozymes in the innate immunity of cyprinid fish, including the structural and phylogenetic characteristics, antimicrobial effects, and regulatory mechanism. |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31402279]; Science and Technology Planning Project of Guangdong Province [2017B030314052, 2014A020208136]; Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding [(2019)75]; Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences [ISEE2018PY03]; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, P.R. China [2017-2A08]; Key Science Project of Lingnan Normal University [LZL1508] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31402279]; Science and Technology Planning Project of Guangdong Province [2017B030314052, 2014A020208136]; Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding [(2019)75]; Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences [ISEE2018PY03]; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, P.R. China [2017-2A08]; Key Science Project of Lingnan Normal University [LZL1508] |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/18518]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Guangdong Inst Drug Control, Microbiol Dept, Guangzhou 510663, Peoples R China 2.Guangdong Haimao Investment Co Ltd, Zhanjiang 524001, Peoples R China 3.Lingnan Normal Univ, Sch Life Sci & Technol, Inst Appl Biotechnol, Zhanjiang 528225, Peoples R China 4.Chinese Acad Sci, Guangdong Prov Key Lab Appl Marine Biol LAMB, CAS Key Lab Trop Marine Bioresources & Ecol LMB, South China Sea Inst Oceanol, Guangzhou 510301, Peoples R China 5.Chinese Acad Sci, Inst South China Sea Ecol & Environm Engn ISEE, Guangzhou 510301, Peoples R China 6.Foshan Univ, Sch Life Sci & Engn, Sch Stomatol & Med, Foshan 528000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Ting,Rao, Yingzhu,Li, Jiaxi,et al. Two Distinct C-Type Lysozymes in Goldfish: Molecular Characterization, Antimicrobial Potential, and Transcriptional Regulation in Response to Opposing Effects of Bacteria/Lipopolysaccharide and Dexamethasone/Leptin[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2020,21(2):501. |
| APA | Chen, Ting.,Rao, Yingzhu.,Li, Jiaxi.,Ren, Chunhua.,Tang, Dongsheng.,...&Yan, Aifen.(2020).Two Distinct C-Type Lysozymes in Goldfish: Molecular Characterization, Antimicrobial Potential, and Transcriptional Regulation in Response to Opposing Effects of Bacteria/Lipopolysaccharide and Dexamethasone/Leptin.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,21(2),501. |
| MLA | Chen, Ting,et al."Two Distinct C-Type Lysozymes in Goldfish: Molecular Characterization, Antimicrobial Potential, and Transcriptional Regulation in Response to Opposing Effects of Bacteria/Lipopolysaccharide and Dexamethasone/Leptin".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21.2(2020):501. |
入库方式： OAI收割
来源：南海海洋研究所
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