Trends in Molecular Markers Associated with Resistance to Sulfadoxine-Pyrimethamine (SP) Among Plasmodium falciparum Isolates on Bioko Island, Equatorial Guinea: 2011-2017
文献类型：期刊论文
| 作者 | Lin, Li-Yun; Li, Jian; Huang, Hui-Ying; Liang, Xue-Yan; Jiang, Ting-Ting; Chen, Jiang-Tao; Ehapo, Carlos Salas3; Eyi, Urbano Monsuy3; Zheng, Yu-Zhong; Zha, Guang-Cai |
| 刊名 | INFECTION AND DRUG RESISTANCE
 |
| 出版日期 | 2020 |
| 卷号 | 13页码:1203 |
| 关键词 | malaria drug resistance dihydrofolate reductase dihydropteroate synthase sulfadoxine-pyrimethamine |
| ISSN号 | 1178-6973 |
| DOI | 10.2147/IDR.S236898 |
| 英文摘要 | Purpose: Antimalarial drug resistance is one of the major challenges in global efforts to control and eliminate malaria. In 2006, sulfadoxine-pyrimethamine (SP) replaced with artemisinin-based combination therapy (ACT) on Bioko Island, Equatorial Guinea, in response to increasing SP resistance, which is associated with mutations in the dihydrofolate reductase (Pfdhfr) and dihydropteroate synthase (Pfdhps) genes. Patients and Methods: To evaluate the trend of molecular markers associated with SP resistance on Bioko Island from 2011 to 2017, 179 samples collected during active case detection were analysed by PCR and DNA sequencing. Results: Pfdhfr and Pfdhps gene sequences were obtained for 90.5% (162/179) and 77.1% (138/179) of the samples, respectively. For Pfdhfr, 97.5% (158/162), 95.7% (155/162) and 98.1% (159/162) of the samples contained N51I, C59R and S108N mutant alleles, respectively. And Pfdhps S436A, A437G, K540E, A581G, and A613S mutations were observed in 25.4% (35/138), 88.4% (122/138), 5.1% (7/138), 1.4% (2/138), and 7.2% (10/138) of the samples, respectively. Two classes of previously described Pfdhfr-Pfdhps haplotypes associated with SP resistance and their frequencies were identified: partial (IRNI-SGKAA, 59.4%) and full (IRNI-SGEAA, 5.5%) resistance. Although no significant difference was observed in different time periods (p>0.05), our study confirmed a slowly increasing trend of the frequencies of these SP-resistance markers in Bioko parasites over the 7 years investigated. Conclusion: The findings reveal the general existence of SP-resistance markers on Bioko Island even after the replacement of SP as a first-line treatment for uncomplicated malaria. Continuous molecular monitoring and additional control efforts in the region are urgently needed. |
| 资助机构 | Natural Science Foundation of Guangdong ProvinceNational Natural Science Foundation of Guangdong Province [2016A03031311, 2018A030307074]; Guangdong Science and Technology Project [2016A030303064]; Foundation for Innovative Research Team of Hubei University of Medicine [FDFR201603]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81802046, 2019]; Guangdong Province Key Discipline Research Project ; Natural Science Foundation of Guangdong ProvinceNational Natural Science Foundation of Guangdong Province [2016A03031311, 2018A030307074]; Guangdong Science and Technology Project [2016A030303064]; Foundation for Innovative Research Team of Hubei University of Medicine [FDFR201603]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81802046, 2019]; Guangdong Province Key Discipline Research Project |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/18519]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Shantou Univ, Dept Med Genet, Med Coll, Shantou, Guangdong, Peoples R China 2.Malabo Reg Hosp, Dept Med Lab, Malabo, Equat Guinea 3.Huizhou Cent Hosp, Dept Med Lab, Huizhou, Guangdong, Peoples R China 4.Chinese Med Aid Team Republ Equatorial Guinea, Guangzhou, Guangdong, Peoples R China 5.Shantou Univ, Chaozhou Peoples Hosp Affiliated, Dept Med Lab, Med Coll, Chaozhou, Guangdong, Peoples R China 6.Hubei Univ Med, Renmin Hosp, Dept Infect Dis, Shiyan, Hubei, Peoples R China 7.Hubei Univ Med, Renmin Hosp, Sch Basic Med Sci, Dept Human Parasitol, Shiyan, Hubei, Peoples R China 8.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou, Peoples R China 9.Univ Chinese Acad Sci, Beijing, Peoples R China 10.Hanshan Normal Univ, Sch Food Engn & Biotechnol, Chaozhou, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, Li-Yun,Li, Jian,Huang, Hui-Ying,et al. Trends in Molecular Markers Associated with Resistance to Sulfadoxine-Pyrimethamine (SP) Among Plasmodium falciparum Isolates on Bioko Island, Equatorial Guinea: 2011-2017[J]. INFECTION AND DRUG RESISTANCE,2020,13:1203, 1212. |
| APA | Lin, Li-Yun.,Li, Jian.,Huang, Hui-Ying.,Liang, Xue-Yan.,Jiang, Ting-Ting.,...&Lin, Min.(2020).Trends in Molecular Markers Associated with Resistance to Sulfadoxine-Pyrimethamine (SP) Among Plasmodium falciparum Isolates on Bioko Island, Equatorial Guinea: 2011-2017.INFECTION AND DRUG RESISTANCE,13,1203. |
| MLA | Lin, Li-Yun,et al."Trends in Molecular Markers Associated with Resistance to Sulfadoxine-Pyrimethamine (SP) Among Plasmodium falciparum Isolates on Bioko Island, Equatorial Guinea: 2011-2017".INFECTION AND DRUG RESISTANCE 13(2020):1203. |
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来源：南海海洋研究所
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