Diversity of oligotrich ciliates (Ciliophora, Spirotrichea) in the northern coast of South China Sea as revealed in LSU rDNA sequences
文献类型：期刊论文
| 作者 | Lu, Kaihui; Liu, Weiwei2; Warren, Alan3; Xu, Yusen; Zhu, Changyu; Zhao, Yan1; Yi, Zhenzhen |
| 刊名 | JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| 出版日期 | 2020 |
| 卷号 | 38期号:1页码:156 |
| 关键词 | diversity low-abundance species oligotrich ciliates operational taxonomic units (OTUs) |
| ISSN号 | 2096-5508 |
| DOI | 10.1007/s00343-019-9021-0 |
| 英文摘要 | Although the taxonomy of oligotrich ciliates has been widely investigated, yet the species diversity remains poorly known. We newly designed a pair of oligotrich-specific LSU rDNA primers covering the 600-bp D1/D2 region, and it was effective for detecting oligotrich species. Using the primers, we constructed the cloning libraries to investigate the species diversity of oligotrichs in the northern coastal waters of the South China Sea. In total, 165 oligotrich sequences were obtained from five widely separated sampling sites. Sixty operational taxonomic units (OTUs) were obtained at 99% similarity threshold, and low-abundance OTUs with no more than two sequences contributed most of these (about 78%). Our findings are consistent with previous morphological studies, Strombidium was found the most abundant and widely distributed genus in this area. In addition, the BLAST search in the NCBI database resulted in 95% OTUs matching with named oligotrich species in similarity below 99%. Therefore, oligotrich morphospecies diversity has been underestimated as low-abundance species, and the LSU rDNA oligotrich sequence database needs to be better promoted. |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31772440, 31430077, 41576124, 31761133001]; Pearl River Science and Technology Nova Program of Guangzhou [201610010162]; Guangdong MEPP Fund [GDOE (2019) A23]; Special Support Program of Guangdong Province ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31772440, 31430077, 41576124, 31761133001]; Pearl River Science and Technology Nova Program of Guangzhou [201610010162]; Guangdong MEPP Fund [GDOE (2019) A23]; Special Support Program of Guangdong Province |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/18522]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Nat Hist Museum, Dept Life Sci, London SW7 5BD, England 2.South China Normal Univ, Lab Protozool, Guangzhou Key Lab Subtrop Biodivers & Biomonitori, Sch Life Sci, Guangzhou 510631, Peoples R China 3.Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Peoples R China 4.Capital Normal Univ, Coll Life Sci, Beijing 100048, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Kaihui,Liu, Weiwei,Warren, Alan,et al. Diversity of oligotrich ciliates (Ciliophora, Spirotrichea) in the northern coast of South China Sea as revealed in LSU rDNA sequences[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2020,38(1):156, 167. |
| APA | Lu, Kaihui.,Liu, Weiwei.,Warren, Alan.,Xu, Yusen.,Zhu, Changyu.,...&Yi, Zhenzhen.(2020).Diversity of oligotrich ciliates (Ciliophora, Spirotrichea) in the northern coast of South China Sea as revealed in LSU rDNA sequences.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,38(1),156. |
| MLA | Lu, Kaihui,et al."Diversity of oligotrich ciliates (Ciliophora, Spirotrichea) in the northern coast of South China Sea as revealed in LSU rDNA sequences".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 38.1(2020):156. |
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来源：南海海洋研究所
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