Molecular characterization of a RNA polymerase (RNAP) II (DNA directed) polypeptide H (POLR2H) in Pacific white shrimp (Litopenaeus vannamei) and its role in response to high-pH stress
文献类型：期刊论文
| 作者 | Luo, Zhi-Zhan; Sun, Hui-Ming; Guo, Jing-Wen; Luo, Peng; Hu, Chao-Qun; Huang, Wen2,7,10; Shu, Hu5 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2020 |
| 卷号 | 96页码:245 |
| 关键词 | Pacific white shrimp High-pH stress Molecular cloning RNA polymerase (RNAP) II (DNA directed) polypeptide |
| ISSN号 | 1050-4648 |
| DOI | 10.1016/j.fsi.2019.12.012 |
| 英文摘要 | RNA polymerase (RNAP) II (DNA-directed) (POLR2) genes are essential for cell viability under environmental stress and for the transfer of biological information from DNA to RNA. However, the function and characteristics of POLR2 genes in crustaceans are still unknown. In the present study, a POLR2H cDNA was isolated from Pacific white shrimp (Litopenaeus vannamei) and designated as Lv-POLR2H. The full-length Lv-POLR2H cDNA is 772 bp in length and contains a 32-bp 5'-untranslated region (UTR), a 284-bp 3'-UTR with a poly (A) sequence, and an open reading frame (ORF) of 456 bp encoding an Lv-POLR2H protein of 151 amino acids with a deduced molecular weight of 17.21 kDa. The Lv-POLR2H protein only contains one functional domain, harbors no transmembrane domains and mainly locates in the nucleus. The expression of the Lv-POLR2H mRNA was ubiquitously detected in all selected tissues, with the highest level in the gills. In situ hybridization (ISH) analysis showed that Lv-POLR2H was mainly located in the secondary gill filaments, the transcript levels of Lv-POLR2H in the gills were found to be significantly affected after challenge by pH, low salinity and high concentrations of NO2- and NH4+, indicating that Lv-POLR2H in gill tissues might play roles under various physical stresses. Specifically, under high-pH stress, knockdown of Lv-POLR2H via siRNA significantly decreased the survival rate of the shrimp, indicating its key roles in the response to high-pH stress. Our study may provide the first evidence of the role of POLR2H in shrimp responding to high-pH stress and provides new insight into molecular regulation in response to high pH in crustaceans. |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31602135]; Natural Science Foundation of Guangdong ProvinceNational Natural Science Foundation of Guangdong Province [2016A030310112]; Science & Technology Promoting Projects for Oceanic & Fishery in Guangdong Province [A201601A03]; Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences [ISEE2018PY03] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31602135]; Natural Science Foundation of Guangdong ProvinceNational Natural Science Foundation of Guangdong Province [2016A030310112]; Science & Technology Promoting Projects for Oceanic & Fishery in Guangdong Province [A201601A03]; Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences [ISEE2018PY03] |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/18525]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.[Luo, Peng 2.Hu, Chao-Qun 3.[Luo, Zhi-Zhan 4.Sun, Hui-Ming 5.Guo, Jing-Wen 6.Guangzhou Univ, Sch Life Sci, Sch Environm Sci & Engn, Guangzhou 510006, Peoples R China 7.[Luo, Zhi-Zhan 8.Guangdong Acad Agr Sci, Inst Anim Sci, Guangzhou 510640, Peoples R China 9.[Luo, Peng 10.Hu, Chao-Qun |
| 推荐引用方式 GB/T 7714 | Luo, Zhi-Zhan,Sun, Hui-Ming,Guo, Jing-Wen,et al. Molecular characterization of a RNA polymerase (RNAP) II (DNA directed) polypeptide H (POLR2H) in Pacific white shrimp (Litopenaeus vannamei) and its role in response to high-pH stress[J]. FISH & SHELLFISH IMMUNOLOGY,2020,96:245, 253. |
| APA | Luo, Zhi-Zhan.,Sun, Hui-Ming.,Guo, Jing-Wen.,Luo, Peng.,Hu, Chao-Qun.,...&Shu, Hu.(2020).Molecular characterization of a RNA polymerase (RNAP) II (DNA directed) polypeptide H (POLR2H) in Pacific white shrimp (Litopenaeus vannamei) and its role in response to high-pH stress.FISH & SHELLFISH IMMUNOLOGY,96,245. |
| MLA | Luo, Zhi-Zhan,et al."Molecular characterization of a RNA polymerase (RNAP) II (DNA directed) polypeptide H (POLR2H) in Pacific white shrimp (Litopenaeus vannamei) and its role in response to high-pH stress".FISH & SHELLFISH IMMUNOLOGY 96(2020):245. |
入库方式： OAI收割
来源：南海海洋研究所
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