LvPPAE2 induced by WSV056 confers host defense against WSSV in Litopenaeus vannamei
文献类型：期刊论文
| 作者 | Wang, Wei; Luo, Peng2; Pan, Changkun; Wang, Qingbai3; Yuan, Huifang4; Liu, Jieping5; Jin, Chunying6; Chen, Jianming; Wu, Wenlin5 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2020 |
| 卷号 | 96页码:319 |
| 关键词 | Litopenaeus vannamei WSV056 Serine protease Prophenoloxidase activating enzyme Innate immunity |
| ISSN号 | 1050-4648 |
| DOI | 10.1016/j.fsi.2019.12.002 |
| 英文摘要 | Viral immediate early (IE) genes encode regulatory proteins that are critical for viral replication. WSV056 is an IE protein of white spot syndrome virus (WSSV), an important pathogen of farmed shrimp. It targets the host Rb protein(s) and, according to a previous study, may enhance the replication of the viral genome. However, the ectopic expression of WSV056 in transgenic Drosophila melanogaster exerted an inhibitory effect on the replication of Drosophila C virus (DCV). Transcriptome study using Affymetrix GeneChip suggested that the enrichment of serine proteases (SPs) likely accounts for DCV inhibition in WSV056-overexpressing Drosophila. Injection of recombinant WSV056 to the WSSV natural host Litopenaeus vannamei enhanced the expression of the SP family member prophenoloxidase-activating enzyme 2 (LvPPAE2) and conferred shrimp with more resistance to WSSV infection. LvPPAE2 knockdown contributed to decreased expression of antimicrobial peptides LvAlf1 and LvLyz1, reduced hemolymph phenoloxidase activity, and increased virus load, suggesting that LvPPAE2 is involved in the host defense against WSSV infection. Taken together, these results suggest that wsv056 plays a role in restricting viral replication by inducing the SP-mediated immune responses in the host. |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31272684, 31470133, 41276176]; Research Program of Minjiang University [MYK18020] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31272684, 31470133, 41276176]; Research Program of Minjiang University [MYK18020] |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/18526]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Huaqiao Univ, Coll Chem Engn, Xiamen 361000, Fujian, Peoples R China 2.Minjiang Univ, Inst Oceanog, Fuzhou 350108, Fujian, Peoples R China 3.Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangdong Prov Key Lab Appl Marine Biol LAMB, South China Sea Inst Oceanol, Guangzhou 501301, Guangdong, Peoples R China 4.Guangdong Acad Sci, Guangdong Inst Microbiol, Guangzhou 501301, Guangdong, Peoples R China 5.Univ Queensland, Sch Biol Sci, Brisbane, Qld 4072, Australia 6.Quanzhou Normal Univ, Coll Oceanol & Food Sci, Quanzhou 362000, Fujian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Wei,Luo, Peng,Pan, Changkun,et al. LvPPAE2 induced by WSV056 confers host defense against WSSV in Litopenaeus vannamei[J]. FISH & SHELLFISH IMMUNOLOGY,2020,96:319, 329. |
| APA | Wang, Wei.,Luo, Peng.,Pan, Changkun.,Wang, Qingbai.,Yuan, Huifang.,...&Wu, Wenlin.(2020).LvPPAE2 induced by WSV056 confers host defense against WSSV in Litopenaeus vannamei.FISH & SHELLFISH IMMUNOLOGY,96,319. |
| MLA | Wang, Wei,et al."LvPPAE2 induced by WSV056 confers host defense against WSSV in Litopenaeus vannamei".FISH & SHELLFISH IMMUNOLOGY 96(2020):319. |
入库方式： OAI收割
来源：南海海洋研究所
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