SmGRAS1 and SmGRAS2 Regulate the Biosynthesis of Tanshinones and Phenolic Acids in Salvia miltiorrhiza
文献类型：期刊论文
| 作者 | Li, Wenrui1,4; Bai, Zhenqing2; Pei, Tianlin2; Yang, Dongfeng3; Mao, Renjun2; Zhang, Bingxue1,4; Liu, Chuangfeng2; Liang, Zongsuo3,4 |
| 刊名 | FRONTIERS IN PLANT SCIENCE
 |
| 出版日期 | 2019-10-30 |
| 卷号 | 10页码:13 |
| 关键词 | Salvia miltiorrhiza SmGRAS1 2 GA tanshinines phenolic acids biosynthesis |
| ISSN号 | 1664-462X |
| DOI | 10.3389/fpls.2019.01367 |
| 通讯作者 | Liang, Zongsuo(liangzs@ms.iswc.ac.cn) |
| 英文摘要 | Salvia miltiorrhiza is one of the most widely used traditional Chinese medicinal plants because of its excellent performance in treating heart diseases. Tanshinones and phenolic acids are two important classes of effective metabolites, and their biosynthesis has attracted widespread interest. Here, we functionally characterized SmGRAS1 and SmGRAS2, two GRAS family transcription factors from S. miltiorrhiza. SmGRAS1/2 were highly expressed in the root periderm, where tanshinones mainly accumulated in S. miltiorrhiza. Overexpression of SmGRAS1/2 upregulated tanshinones accumulation and downregulated GA, phenolic acids contents, and root biomass. However, antisense expression of SmGRAS1/2 reduced the tanshinones accumulation and increased the GA, phenolic acids contents, and root biomass. The expression patterns of biosynthesis genes were consistent with the changes in compounds accumulation. GA treatment increased tanshinones, phenolic acids, and GA contents in the overexpression lines, and restored the root growth inhibited by overexpressing SmGRAS1/2. Subsequently, yeast one-hybrid, dual-luciferase, and electrophoretic mobility shift assays (EMSA) showed SmGRAS1 promoted tanshinones biosynthesis by directly binding to the GARE motif in the SmKSL1 promoter and activating its expression. Yeast two-hybrid assays showed SmGRAS1 interacted physically with SmGRAS2. Taken together, the results revealed that SmGRAS1/2 acted as repressors in root growth and phenolic acids biosynthesis but as positive regulators in tanshinones biosynthesis. Overall, our findings revealed the potential value of SmGRAS1/2 in genetically engineering changes in secondary metabolism. |
| WOS关键词 | HAIRY ROOTS ; GIBBERELLIN ; GENE ; ACCUMULATION ; EXPRESSION ; MECHANISM ; PATHWAY ; FAMILY ; MODULE ; GROWTH |
| 资助项目 | National Natural Science Foundation of China[81373908] ; Ministry of Science and Technology of the People's Republic of China through the Twelfth Five-Year National Science and Technology Pillar Program[2015BAC01B03] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000496167000001 |
| 出版者 | FRONTIERS MEDIA SA |
| 资助机构 | National Natural Science Foundation of China ; Ministry of Science and Technology of the People's Republic of China through the Twelfth Five-Year National Science and Technology Pillar Program |
| 源URL | [http://ir.igsnrr.ac.cn/handle/311030/131844]  |
| 专题 | 中国科学院地理科学与资源研究所 |
| 通讯作者 | Liang, Zongsuo |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Northwest A&F Univ, Coll Life Sci, Yangling, Shaanxi, Peoples R China 3.Zhejiang Sci Tech Univ, Coll Life Sci & Med, Hangzhou, Zhejiang, Peoples R China 4.Chinese Acad Sci & Minist Water Resources, Inst Soil & Water Conservat, Yangling, Shaanxi, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Wenrui,Bai, Zhenqing,Pei, Tianlin,et al. SmGRAS1 and SmGRAS2 Regulate the Biosynthesis of Tanshinones and Phenolic Acids in Salvia miltiorrhiza[J]. FRONTIERS IN PLANT SCIENCE,2019,10:13. |
| APA | Li, Wenrui.,Bai, Zhenqing.,Pei, Tianlin.,Yang, Dongfeng.,Mao, Renjun.,...&Liang, Zongsuo.(2019).SmGRAS1 and SmGRAS2 Regulate the Biosynthesis of Tanshinones and Phenolic Acids in Salvia miltiorrhiza.FRONTIERS IN PLANT SCIENCE,10,13. |
| MLA | Li, Wenrui,et al."SmGRAS1 and SmGRAS2 Regulate the Biosynthesis of Tanshinones and Phenolic Acids in Salvia miltiorrhiza".FRONTIERS IN PLANT SCIENCE 10(2019):13. |
入库方式： OAI收割
来源：地理科学与资源研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。