SmGRAS3 negatively responds to GA signaling while promotes tanshinones biosynthesis in Salvia miltiorrhiza
文献类型：期刊论文
| 作者 | Li, Wenrui1,2; Xing, Bingcong1,2; Mao, Renjun3; Bai, Zhenqing3; Yang, Dongfeng4; Xu, Jiayi3; Liang, Zongsuo1,4 |
| 刊名 | INDUSTRIAL CROPS AND PRODUCTS
 |
| 出版日期 | 2020-02-01 |
| 卷号 | 144页码:11 |
| ISSN号 | 0926-6690 |
| 关键词 | Salvia mildorrhiza Tanshinones GRAS GA signaling Regulation |
| DOI | 10.1016/j.indcrop.2019.112004 |
| 通讯作者 | Liang, Zongsuo(liangzs@ms.iswc.ac.cn) |
| 英文摘要 | Salvia miltiorrhiza Bunge is the origin plant of traditional Chinese medicine, Danshen. Demand of high-quality S. miltiorrhiza is increasing because of its excellent pharmaceutical value. Tanshinones and salvianolic acids (SAs) are the main active ingredients of S. miltiorrhiza. GA was reported to induce tanshinones and SAs accumulation, however, its mechanism was unclear. Here, we got a GA responsive protein, SmGRAS3. SmGRAS3 expressed highest in the periderm where tanshinones produced and accumulated. Results of SmGRAS3 over and antisense expression indicated that SmGRAS3 is a positive regulator of tanshinones biosynthesis in S. miltiorrhiza hairy roots. But overexpression of SmGRAS3 depressed SAs and GA biosynthesis as well as hairy roots growth, and application of GA could partially recover these depressions. Pathway enzyme genes expressions of tanshinones, SAs and GA biosynthesis were comprehensively changed in SmGRAS3 transgenic hairy roots. SmGRAS3 acted as a transcription factor that located in nucleus and has the transcription activation activity. Yeast one-hybrid (Y1H) and electrophoretic mobility shift assay (EMSA) showed that SmGRAS3 directly bound to GARE-motif in promoter of KSL1 and activated KSL1 transcription. Our study suggested that the SmGRAS3 may be a good potential target for further metabolic engineering of bioactive components biosynthesis in S. miltiorrhiza. |
| WOS关键词 | CHINESE MEDICINAL PREPARATIONS ; GRAS TRANSCRIPTION FACTOR ; HAIRY ROOT CULTURES ; PHENOLIC-ACIDS ; SYNTHASE GENE ; EXPRESSION ; ACCUMULATION ; SCARECROW ; PROTEINS ; INTERACTS |
| 资助项目 | National Natural Science Foundation of China[81373908] ; Ministry of Science and Technology of the People's Republic of China through the Twelfth Five-Year National Science and Technology Pillar Program[2015BACO1B03] |
| WOS研究方向 | Agriculture |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000510521700010 |
| 资助机构 | National Natural Science Foundation of China ; Ministry of Science and Technology of the People's Republic of China through the Twelfth Five-Year National Science and Technology Pillar Program |
| 源URL | [http://ir.igsnrr.ac.cn/handle/311030/132222]  |
| 专题 | 中国科学院地理科学与资源研究所 |
| 通讯作者 | Liang, Zongsuo |
| 作者单位 | 1.Chinese Acad Sci & Minist Water Resources, Inst Soil & Water Conservat, Yangling 712100, Shaanxi, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China 4.Zhejiang Sci Tech Univ, Coll Life Sci & Med, Hangzhou 310018, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Wenrui,Xing, Bingcong,Mao, Renjun,et al. SmGRAS3 negatively responds to GA signaling while promotes tanshinones biosynthesis in Salvia miltiorrhiza[J]. INDUSTRIAL CROPS AND PRODUCTS,2020,144:11. |
| APA | Li, Wenrui.,Xing, Bingcong.,Mao, Renjun.,Bai, Zhenqing.,Yang, Dongfeng.,...&Liang, Zongsuo.(2020).SmGRAS3 negatively responds to GA signaling while promotes tanshinones biosynthesis in Salvia miltiorrhiza.INDUSTRIAL CROPS AND PRODUCTS,144,11. |
| MLA | Li, Wenrui,et al."SmGRAS3 negatively responds to GA signaling while promotes tanshinones biosynthesis in Salvia miltiorrhiza".INDUSTRIAL CROPS AND PRODUCTS 144(2020):11. |
入库方式： OAI收割
来源：地理科学与资源研究所
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