Soil enzyme kinetics indicate ecotoxicity of long-term arsenic pollution in the soil at field scale
文献类型：期刊论文
| 作者 | Wang, Ziquan2,3; Tian, Haixia2; Lei, Mei4 ; Megharaj, Mallavarapu5; Tan, Xiangping1,2; Wang, Fang3,6; Jia, Hanzhong2; He, Wenxiang2
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| 刊名 | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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| 出版日期 | 2020-03-15 |
| 卷号 | 191页码:8 |
| 关键词 | Field soil pollution Catalytic efficiency Risk assessment Bioindicator Ecological dose |
| ISSN号 | 0147-6513 |
| DOI | 10.1016/j.ecoenv.2020.110215 |
| 通讯作者 | Jia, Hanzhong(jiahz@nwafu.edu.cn) ; He, Wenxiang(wenxiang.he@nwafu.edu.cn) |
| 英文摘要 | Information on the kinetic characteristics of soil enzymes under long-term arsenic (As) pollution in field soils is scarce. We investigated Michaelis-Menten kinetic properties of four soil enzymes including beta-glucosidase (BG), acid phosphatase (ACP), alkaline phosphatase (ALP), and dehydrogenase (DHA) in field soils contaminated by As resulting from long-term realgar mining activity. The kinetic parameters, namely the maximum reaction velocity (V-max), enzyme-substrate affinity (K-m) and catalytic efficiency (V-max/K-m) were calculated. Results revealed that the enzyme kinetic characteristics varied in soils and were significantly influenced by total nitrogen (N) and total As, which explained 31.8% and 30.7% of the variance in enzyme kinetics respectively. Enzyme pools (V-max) and catalytic efficiency (V-max/K-m) of BG, ACP and DHA decreased with elevated As pollution, while the enzyme affinity for substrate (K-m) was less affected. Redundancy analysis and stepwise regression suggested that the adverse influence of As on enzyme kinetics may offset or weakened by soil total N and soil organic matter (SOM). Concentration-response fitting revealed that the specific kinetic parameters expressed as the absolute enzyme kinetic parameters multiplied by normalized soil total N and SOM were more relevant than the absolute ones to soil total As. The arsenic ecological dose values that cause 10% decrease (ED10) in the specific enzyme kinetics were 20-49 mg kg(-1), with a mean value of 35 mg kg(-1), indicating a practical range of threshold for As contamination at field level. This study concluded that soil enzymes exhibited functional adaptation to long-term As stress mainly through the reduction of enzyme pools (Vmax) or maintenance of enzyme-substrate affinity (K-m). Further, this study demonstrates that the specific enzyme kinetics are the better indicators of As ecotoxicity at field-scale compared with the absolute enzyme parameters. |
| WOS关键词 | ALKALINE-PHOSPHATASE ; MICROBIAL COMMUNITY ; DEHYDROGENASE-ACTIVITY ; CATALYTIC EFFICIENCY ; HEAVY-METALS ; CONTAMINATION ; TOXICITY ; AVAILABILITY ; REMEDIATION ; MANAGEMENT |
| 资助项目 | National Natural Science Foundation of China[41571245] ; National Natural Science Foundation of China[41603116] ; National Natural Science Foundation of China[31600384] ; Basic Scientific Research Foundation of Northwest A F University[ZD2013012] ; China Postdoctoral Science Foundation[2016M592549] |
| WOS研究方向 | Environmental Sciences & Ecology ; Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:000514011400011 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 资助机构 | National Natural Science Foundation of China ; Basic Scientific Research Foundation of Northwest A F University ; China Postdoctoral Science Foundation |
| 源URL | [http://ir.igsnrr.ac.cn/handle/311030/132834]  |
| 专题 | 中国科学院地理科学与资源研究所 |
| 通讯作者 | Jia, Hanzhong; He, Wenxiang |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Vegetat Restorat & Management Degraded Ec, South China Bot Garden, 723 Xingke Rd, Guangzhou 510650, Peoples R China 2.Northwest A&F Univ, Coll Nat Resources & Environm, Key Lab Plant Nutr & Agroenvironm Northwest China, Minist Agr, Yangling 712100, Shaanxi, Peoples R China 3.Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Peoples R China 4.Chinese Acad Sci, Ctr Environm Remediat, Inst Geog Sci & Nat Resources Res, Beijing 100101, Peoples R China 5.Univ Newcastle, Fac Sci, Global Ctr Environm Remediat, Callaghan, NSW 2308, Australia 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Ziquan,Tian, Haixia,Lei, Mei,et al. Soil enzyme kinetics indicate ecotoxicity of long-term arsenic pollution in the soil at field scale[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2020,191:8. |
| APA | Wang, Ziquan.,Tian, Haixia.,Lei, Mei.,Megharaj, Mallavarapu.,Tan, Xiangping.,...&He, Wenxiang.(2020).Soil enzyme kinetics indicate ecotoxicity of long-term arsenic pollution in the soil at field scale.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,191,8. |
| MLA | Wang, Ziquan,et al."Soil enzyme kinetics indicate ecotoxicity of long-term arsenic pollution in the soil at field scale".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 191(2020):8. |
入库方式： OAI收割
来源：地理科学与资源研究所
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