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Light regulation of resistance to oxidative damage and magnetic crystal biogenesis inMagnetospirillum magneticummediated by a Cys-less LOV-like protein

文献类型：期刊论文

作者Chen, Haitao2,3,4; Li, Kefeng2,5; Cai, Yao6; Wang, Pingping2,4; Gong, Weimin1; Wu, Long-Fei4,7; Song, Tao2,3,4
刊名APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
出版日期2020-08-11
页码15
关键词LOV Magnetotactic bacteria Amb2291 Oxidative stress Reactive oxygen species
ISSN号0175-7598
DOI10.1007/s00253-020-10807-5
英文摘要Light-oxygen-voltage (LOV) proteins are ubiquitous photoreceptors that can interact with other regulatory proteins and then mediate their activities, which results in cellular adaptation and subsequent physiological changes. Upon blue-light irradiation, a conserved cysteine (Cys) residue in LOV covalently binds to flavin to form a flavin-Cys adduct, which triggers a subsequent cascade of signal transduction and reactions. We found a group of natural Cys-less LOV-like proteins in magnetotactic bacteria (MTB) and investigated its physiological functions by conducting research on one of these unusual LOV-like proteins, Amb2291, inMagnetospirillum magneticum. In-frame deletion ofamb2291or site-directive substitution of alanine-399 for Cys mutants impaired the protective responses against hydrogen peroxide, thereby causing stress and growth impairment. Consequently, gene expression and magnetosome formation were affected, which led to high sensitivity to oxidative damage and defective phototactic behaviour. The purified wild-type and A399C-mutated LOV-like proteins had similar LOV blue-light response spectra, but Amb2291(A399C)exhibited a faster reaction to blue light. We especially showed that LOV-like protein Amb2291 plays a role in magnetosome synthesis and resistance to oxidative stress of AMB-1 when this bacterium was exposed to red light and hydrogen peroxide. This finding expands our knowledge of the physiological function of this widely distributed group of photoreceptors and deepens our understanding of the photoresponse of MTB.
WOS关键词BLUE-LIGHT ; MAGNETOTACTIC BACTERIA ; MOLECULAR-MECHANISMS ; INTEGRATING LIGHT ; DOMAIN PROTEIN ; SINGLET OXYGEN ; STRESS ; KINASE ; PHOTOTROPIN ; MAGNETOSOMES
资助项目StateKey Program of National Natural Science of China[51937011] ; Institute of Electrical Engineering, Chinese Academy of Sciences[Y650141CSA]
WOS研究方向Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:000558596600002
出版者SPRINGER
资助机构StateKey Program of National Natural Science of China ; StateKey Program of National Natural Science of China ; Institute of Electrical Engineering, Chinese Academy of Sciences ; Institute of Electrical Engineering, Chinese Academy of Sciences ; StateKey Program of National Natural Science of China ; StateKey Program of National Natural Science of China ; Institute of Electrical Engineering, Chinese Academy of Sciences ; Institute of Electrical Engineering, Chinese Academy of Sciences ; StateKey Program of National Natural Science of China ; StateKey Program of National Natural Science of China ; Institute of Electrical Engineering, Chinese Academy of Sciences ; Institute of Electrical Engineering, Chinese Academy of Sciences ; StateKey Program of National Natural Science of China ; StateKey Program of National Natural Science of China ; Institute of Electrical Engineering, Chinese Academy of Sciences ; Institute of Electrical Engineering, Chinese Academy of Sciences
源URL[http://ir.iggcas.ac.cn/handle/132A11/97626]  
专题地质与地球物理研究所_中国科学院地球与行星物理重点实验室
通讯作者Wu, Long-Fei; Song, Tao
作者单位1.Univ Sci & Technol China, Sch Life Sci, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Peoples R China
2.Chinese Acad Sci, Inst Elect Engn, Beijing Key Lab Biol Electromagnetism, Beijing 100190, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.CNRS CAS, France China Int Lab Evolut & Dev Magnetotact Mul, Beijing 100190, Peoples R China
5.Shandong Sport Univ, Jinan 250102, Peoples R China
6.Chinese Acad Sci, Inst Geol & Geophys, Key Lab Earth & Planetary Phys, Beijing 100029, Peoples R China
7.Aix Marseille Univ, LCB, CNRS, F-13402 Marseille, France
推荐引用方式
GB/T 7714		 Chen, Haitao,Li, Kefeng,Cai, Yao,et al. Light regulation of resistance to oxidative damage and magnetic crystal biogenesis inMagnetospirillum magneticummediated by a Cys-less LOV-like protein[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2020:15.
APA Chen, Haitao.,Li, Kefeng.,Cai, Yao.,Wang, Pingping.,Gong, Weimin.,...&Song, Tao.(2020).Light regulation of resistance to oxidative damage and magnetic crystal biogenesis inMagnetospirillum magneticummediated by a Cys-less LOV-like protein.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,15.
MLA Chen, Haitao,et al."Light regulation of resistance to oxidative damage and magnetic crystal biogenesis inMagnetospirillum magneticummediated by a Cys-less LOV-like protein".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2020):15.

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来源：地质与地球物理研究所

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