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Synthesis, characterization and application of magnetoferritin nanoparticle by using human H chain ferritin expressed byPichia pastoris

文献类型：期刊论文

作者Cai, Yao1,2,7; Huang, Jinjin3,4,5; Xu, Huangtao1,2,6,7; Zhang, Tongwei1,2,7; Cao, Changqian1,2,7; Pan, Yongxin1,2,6,7
刊名NANOTECHNOLOGY
出版日期2020-11-27
卷号31期号:48页码:11
ISSN号0957-4484
关键词human H chain of ferritin Pichia pastoris magnetoferritin imaging
DOI10.1088/1361-6528/abb15d
英文摘要Protein-based nanoparticles have developed rapidly in areas such as drug delivery, biomedical imaging and biocatalysis. Ferritin possesses unique properties that make it attractive as a potential platform for a variety of nanobiotechnological applications. Here we synthesized magnetoferritin (P-MHFn) nanoparticles for the first time by using the human H chain of ferritin that was expressed byPichia pastoris(P-HFn). Western blot results showed that recombinant P-HFn was successfully expressed after methanol induction. Transmission electron microscopy (TEM) showed the spherical cage-like shape and monodispersion of P-HFn. The synthesized magnetoferritin (P-MHFn) retained the properties of magnetoferritin nanoparticles synthesized using HFn expressed byE. coli(E-MHFn): superparamagnetism under ambient conditions and peroxidase-like activity. It is stable under a wider range of pH values (from 5.0 to 11.0), likely due to post-translational modifications such as N-glycosylation on P-HFn.In vivonear-infrared fluorescence imaging experiments revealed that P-MHFn nanoparticles can accumulate in tumors, which suggests that P-MHFn could be used in tumor imaging and therapy. An acute toxicity study of P-MHFn in Sprague Dawley rats showed no abnormalities at a dose up to 20 mg Fe Kg(-1)body weight. Therefore, this study shed light on the development of magnetoferritin nanoparticles using therapeutic HFn expressed byPichia pastorisfor biomedical applications.
WOS关键词PROTEIN EXPRESSION ; NANOCAGES ; BINDING ; CAGES
资助项目National Natural Science Foundation of China[41704072] ; National Natural Science Foundation of China[41621004] ; China Postdoctoral Science Foundation[2017M610979] ; Key Program of Chinese Academy of Sciences[QYZDJ-SSWDQC024]
WOS研究方向Science & Technology - Other Topics ; Materials Science ; Physics
语种英语
出版者IOP PUBLISHING LTD
WOS记录号WOS:000570413100001
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Key Program of Chinese Academy of Sciences ; Key Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Key Program of Chinese Academy of Sciences ; Key Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Key Program of Chinese Academy of Sciences ; Key Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Key Program of Chinese Academy of Sciences ; Key Program of Chinese Academy of Sciences
源URL[http://ir.iggcas.ac.cn/handle/132A11/98254]  
专题地质与地球物理研究所_中国科学院地球与行星物理重点实验室
通讯作者Cai, Yao; Pan, Yongxin
作者单位1.Chinese Acad Sci, France China Int Lab Evolut & Dev Magnetotact Mul, Beijing 100029, Peoples R China
2.Chinese Acad Sci, Biogeomagnetism Grp, Key Lab Earth & Planetary Phys, Inst Geol & Geophys, Beijing 100029, Peoples R China
3.China Agr Univ, MOA Key Lab Soil Microbiol, Coll Biol Sci, Beijing 100193, Peoples R China
4.China Agr Univ, State Key Lab Agrobiotechnol, Coll Biol Sci, Beijing 100193, Peoples R China
5.Jiangsu Normal Univ, Key Lab Biotechnol Med Plant Jiangsu Prov, Sch Life Sci, Xuzhou 221116, Jiangsu, Peoples R China
6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
7.Chinese Acad Sci, Innovat Acad Earth Sci, Beijing 100029, Peoples R China
推荐引用方式
GB/T 7714		 Cai, Yao,Huang, Jinjin,Xu, Huangtao,et al. Synthesis, characterization and application of magnetoferritin nanoparticle by using human H chain ferritin expressed byPichia pastoris[J]. NANOTECHNOLOGY,2020,31(48):11.
APA Cai, Yao,Huang, Jinjin,Xu, Huangtao,Zhang, Tongwei,Cao, Changqian,&Pan, Yongxin.(2020).Synthesis, characterization and application of magnetoferritin nanoparticle by using human H chain ferritin expressed byPichia pastoris.NANOTECHNOLOGY,31(48),11.
MLA Cai, Yao,et al."Synthesis, characterization and application of magnetoferritin nanoparticle by using human H chain ferritin expressed byPichia pastoris".NANOTECHNOLOGY 31.48(2020):11.

入库方式： OAI收割

来源：地质与地球物理研究所

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