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Gadolinium-Labeled Ferritin Nanoparticles as T-1 Contrast Agents for Magnetic Resonance Imaging of Tumors

文献类型：期刊论文

作者Cai, Yao2,3,4; Wang, Yuqing1; Zhang, Tongwei2,3,4; Pan, Yongxin2,3,4
刊名ACS APPLIED NANO MATERIALS
出版日期2020-09-25
卷号3期号:9页码:8771-8783
ISSN号2574-0970
关键词ferritin contrast agents T-1-weighted magnetic resonance imaging tumor targeting low dose
DOI10.1021/acsanm.0c01563
英文摘要Recombinant human heavy chain (H-chain) ferritin (HFn) with gadolinium labeling (HFn-Gd) was designed as a targeted T1 contrast agent for magnetic resonance imaging (MRI) to enhance MRI efficiency in diagnosing tumors at a low dosage. The HFn-Gd nanoparticle shows a larger hydrodynamic diameter (13.6 nm) as compared to HFn (12.5 nm), and a decreased zeta potential value (-14.3 mv) as compared to HFn (-6.2 mv). Fourier transform infrared spectroscopy characterization and X-ray photoelectron spectroscopy analysis indicate the conjugation of Gd on HFn nanoparticles. Compared with Gd-DTPA (DTPA, diethylenetriaminepentaacetic acid), the HFn-Gd nanoparticle has a high r(1) value of 4.78 mM(-1) s(-1), showing good biocompatibility, stability, and active targeting ability to TfR1-positive cancer cells as revealed by cell viability tests, arsenazo III colorimetric assays, and laser scanning confocal microscopy examinations. The MRI of CFPAC-1 tumors using HFn-Gd nanoparticles (dose = 0.016 mmol Gd/kg body weight) shows an enhanced signal up to 30 min postinjection, which is significantly longer than that of Gd-DTPA (approximately 10 min). At such a low dose, the MRI of microscopic MDA-MB-231 tumors, with HFn-Gd nanoparticles, shows a significant signal change up to 60 min, while almost no detectable signal change occurs with Gd-DTPA. Furthermore, MRI reveals that HFn-Gd nanoparticles are mostly cleared through the renal system. Our results demonstrate that the HFn protein-based contrast agent integrates T-1 signal enhancing, precise tumor targeting, and systematic clearance capabilities. It is an effective T-1 contrast agent for MR imaging of tumors with a single low-dose injection.
WOS关键词IRON-OXIDE NANOPARTICLES ; DRUG-DELIVERY ; HIGH RELAXIVITY ; H-FERRITIN ; DEPOSITION ; NANOPROBES ; NANOCAGES ; COMPLEX ; RGD
资助项目National Natural Science Foundation of China (NSFC) ; China Postdoctoral Science Foundation ; Chinese Academy of Sciences ; NSFC[41704072] ; NSFC[41621004] ; China Postdoctoral Science Foundation[2017M610979] ; Chinese Academy of Sciences[QYZDJ-SSWDQC024]
WOS研究方向Science & Technology - Other Topics ; Materials Science
语种英语
出版者AMER CHEMICAL SOC
WOS记录号WOS:000575846000025
资助机构National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; NSFC ; NSFC ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; NSFC ; NSFC ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; NSFC ; NSFC ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; NSFC ; NSFC ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences
源URL[http://ir.iggcas.ac.cn/handle/132A11/98292]  
专题地质与地球物理研究所_中国科学院地球与行星物理重点实验室
通讯作者Cai, Yao; Pan, Yongxin
作者单位1.Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Innovat Acad Earth Sci, Beijing 100029, Peoples R China
3.Chinese Acad Sci, France China Int Lab Evolut & Dev Magnetotact Mul, Beijing 100029, Peoples R China
4.Chinese Acad Sci, Biogeomagnetism Grp, Key Lab Earth & Planetary Phys, Inst Geol & Geophys, Beijing 100029, Peoples R China
推荐引用方式
GB/T 7714		 Cai, Yao,Wang, Yuqing,Zhang, Tongwei,et al. Gadolinium-Labeled Ferritin Nanoparticles as T-1 Contrast Agents for Magnetic Resonance Imaging of Tumors[J]. ACS APPLIED NANO MATERIALS,2020,3(9):8771-8783.
APA Cai, Yao,Wang, Yuqing,Zhang, Tongwei,&Pan, Yongxin.(2020).Gadolinium-Labeled Ferritin Nanoparticles as T-1 Contrast Agents for Magnetic Resonance Imaging of Tumors.ACS APPLIED NANO MATERIALS,3(9),8771-8783.
MLA Cai, Yao,et al."Gadolinium-Labeled Ferritin Nanoparticles as T-1 Contrast Agents for Magnetic Resonance Imaging of Tumors".ACS APPLIED NANO MATERIALS 3.9(2020):8771-8783.

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来源：地质与地球物理研究所

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