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Identification of carbohydrate peripheral epitopes important for recognition by positive-ion MALDI multistage mass spectrometry

文献类型：期刊论文

作者Huang, Chuncui2; Sun, Shiwei3,4; Yan, Jingyu1; Wang, Hui3; Zhou, Jinyu2; Gao, Huanyu2; Xie, Wenchun2; Li, Yan2,4; Chai, Wengang5
刊名CARBOHYDRATE POLYMERS
出版日期2020-02-01
卷号229页码:13
关键词Oligosaccharide Carbohydrate epitope Blood-group antigen Lewis antigen MALDI-MS
ISSN号0144-8617
DOI10.1016/j.carbpol.2019.115528
英文摘要Carbohydrate sequences are important for various biological processes. It has recently been estimated to have 100,000-500,000 carbohydrate structures in mammalian glycome. However, the peripheral carbohydrate determinants on N- and O-glycoproteins, glycolipids, polysaccharides and secreted free sugars are limited in numbers. Among these blood-group-related antigens the ABO(H)- and Lewis-types are particularly important. Negative-ion MS/MS has been successfully used in assignment of these epitopes on free reducing sugars but cannot be applied to reduced sugars, e.g. O-glycans typically released from mucins as alditols, or in positive-ion detection of either reducing or reduced oligosaccharides. In the present study, we investigate the fragmentation features of permethylated reducing and reduced sugars under positive-ion conditions of multi-stage MALDI-MS, and propose the concept of epitope ion and epitope spectrum for determination of peripheral blood-group related epitopes on secreted human milk oligosaccharides and N-glycans as reducing sugars and O-glycans as reduced alditols in conjunction with MALDI-MS glycan profiling.
资助项目National Natural Science Foundation of China[31600650] ; National Natural Science Foundation of China[31671369] ; National Natural Science Foundation of China[31770775] ; March of Dimes Prematurity Research Center[22-FY18-821]
WOS研究方向Chemistry ; Polymer Science
语种英语
WOS记录号WOS:000501796600061
出版者ELSEVIER SCI LTD
源URL[http://119.78.100.204/handle/2XEOYT63/14990]  
专题中国科学院计算技术研究所期刊论文_英文
通讯作者Li, Yan; Chai, Wengang
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Inst Biophys, 15 Datun Rd, Beijing 100101, Peoples R China
3.Chinese Acad Sci, Inst Comp Technol, Key Lab Intelligent Informat Proc, 6 Kexueyuan South Rd, Beijing 100080, Peoples R China
4.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China
5.Imperial Coll London, Glycosci Lab, Fac Med, Hammersmith Campus, London W12 0NN, England
推荐引用方式
GB/T 7714		 Huang, Chuncui,Sun, Shiwei,Yan, Jingyu,et al. Identification of carbohydrate peripheral epitopes important for recognition by positive-ion MALDI multistage mass spectrometry[J]. CARBOHYDRATE POLYMERS,2020,229:13.
APA Huang, Chuncui.,Sun, Shiwei.,Yan, Jingyu.,Wang, Hui.,Zhou, Jinyu.,...&Chai, Wengang.(2020).Identification of carbohydrate peripheral epitopes important for recognition by positive-ion MALDI multistage mass spectrometry.CARBOHYDRATE POLYMERS,229,13.
MLA Huang, Chuncui,et al."Identification of carbohydrate peripheral epitopes important for recognition by positive-ion MALDI multistage mass spectrometry".CARBOHYDRATE POLYMERS 229(2020):13.

入库方式： OAI收割

来源：计算技术研究所

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