Live-SIMBA: an ImageJ plug-in for the universal and accelerated single molecule-guided Bayesian localization super resolution microscopy (SIMBA) method
文献类型：期刊论文
| 作者 | Li, Hongjia1,2; Xu, Fan3; Gao, Shan1,2; Zhang, Mingshu4; Xue, Fudong4; Xu, Pingyong2,4,5; Zhang, Fa1,2 |
| 刊名 | BIOMEDICAL OPTICS EXPRESS
 |
| 出版日期 | 2020-10-01 |
| 卷号 | 11期号:10页码:5842-5859 |
| ISSN号 | 2156-7085 |
| DOI | 10.1364/BOE.404820 |
| 英文摘要 | Live-cell super-resolution fluorescence microscopy techniques allow biologists to observe subcellular structures, interactions and dynamics at the nanoscale level. Among of them, single molecule-guided Bayesian localization super resolution microscopy (SIMBA) and its derivatives produce an appropriate 50 nm spatial resolution and a 0.1-2s temporal resolution in living cells with simple off-the-shelf total internal reflection fluorescence (TIRF) equipment. However, SIMBA and its derivatives are limited by the requirement for dual-channel dataset or single-channel dataset with special design, the time-consuming calculation for extended field of view and the lack of real-time visualization tool. Here, we propose a universal and accelerated SIMBA ImageJ plug-in, Live-SIMBA, for time-series analysis in living cells. Live-SIMBA circumvents the requirement of dual-channel dataset using intensity-based sampling algorithm and improves the computing speed using multi-core parallel computing technique. Live-SIMBA also better resolves the weak signals inside the specimens with adjustable background estimation and distance-threshold filter. With improved fidelity on reconstructed structures, greatly accelerated computation, and real-time visualization, Live-SIMBA demonstrates its extended capabilities in live-cell super-resolution imaging. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement |
| 资助项目 | National Key Research and Development Program of China[2017YFA0504702] ; National Key Research and Development Program of China[2017YFA0505300] ; National Key Research and Development Program of China[2017YFE0103900] ; National Natural Science Foundation of China[21778069] ; National Natural Science Foundation of China[31421002] ; National Natural Science Foundation of China[61672493] ; National Natural Science Foundation of China[61932018] ; National Natural Science Foundation of China[U1611261] ; National Natural Science Foundation of China[U1611263] ; Beijing Municipal Natural Science Foundation[L182053] ; Project of the National Laboratory of Biomacromolecules |
| WOS研究方向 | Biochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| WOS记录号 | WOS:000577572500033 |
| 出版者 | OPTICAL SOC AMER |
| 源URL | [http://119.78.100.204/handle/2XEOYT63/15704]  |
| 专题 | 中国科学院计算技术研究所期刊论文_英文 |
| 通讯作者 | Xu, Pingyong |
| 作者单位 | 1.Chinese Acad Sci, High Performance Comp Res Ctr, Inst Comp Technol, 6 Kexueyuan South Rd, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47907 USA 4.Chinese Acad Sci, Inst Biophys, Key Lab RNA Biol, Beijing 100101, Peoples R China 5.Chinese Acad Sci, CAS Ctr Excellence Biomacromol, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Hongjia,Xu, Fan,Gao, Shan,et al. Live-SIMBA: an ImageJ plug-in for the universal and accelerated single molecule-guided Bayesian localization super resolution microscopy (SIMBA) method[J]. BIOMEDICAL OPTICS EXPRESS,2020,11(10):5842-5859. |
| APA | Li, Hongjia.,Xu, Fan.,Gao, Shan.,Zhang, Mingshu.,Xue, Fudong.,...&Zhang, Fa.(2020).Live-SIMBA: an ImageJ plug-in for the universal and accelerated single molecule-guided Bayesian localization super resolution microscopy (SIMBA) method.BIOMEDICAL OPTICS EXPRESS,11(10),5842-5859. |
| MLA | Li, Hongjia,et al."Live-SIMBA: an ImageJ plug-in for the universal and accelerated single molecule-guided Bayesian localization super resolution microscopy (SIMBA) method".BIOMEDICAL OPTICS EXPRESS 11.10(2020):5842-5859. |
入库方式： OAI收割
来源：计算技术研究所
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