Transcriptomics reveals the molecular processes of light-induced rapid darkening of the non-obligate cave dweller Oreolalax rhodostigmatus (Megophryidae, Anura) and their genetic basis of pigmentation strategy
文献类型：期刊论文
| 作者 | Zhu, Wei1; Liu, Lusha1; Wang, Xungang1,2; Gao, Xinyu1; Jiang, Jianping1; Wang, Bin1 |
| 刊名 | BMC GENOMICS
 |
| 出版日期 | 2019-05-31 |
| 卷号 | 19页码:422 |
| 关键词 | Cave dweller Melanocortin 1 receptor Pigment regression Melanogenesis Morphological color change |
| ISSN号 | 1471-2164 |
| DOI | 10.1186/s12864-018-4790-y |
| 产权排序 | 1 |
| 文献子类 | Article |
| 英文摘要 | Background: Vertebrates use different pigmentation strategies to adapt to various environments. A large amount of research has been done on disclosing the mechanisms of pigmentation strategies in vertebrates either under light, or, living in constant darkness. However, less attention has been paid to non-obligate, darkness dwellers. Red-spotted toothed toads Oreolalax rhodostigmatus (Megophryidae; Anura) from the karst mountainous region of southwestern China are non-obligate cave dwellers. Most tadpoles of the species possess transparent skin as they inhabit the dark karst caves. But remarkably, the transparent tadpoles can darken just within 15 h once exposed to light. Obviously, it is very significant to reveal molecular mechanisms of the unexpected rapid-darkening phenomenon. Results: We compared the transcriptomes of O. rhodostigmatus tadpoles with different durations of light exposure to investigate the cellular processes and potential regulation signals for their light-induced rapid darkening. Genes involved in melanogenesis (i.e. TYR, TYRP1 and DCT) and melanocyte proliferation, as well as their transcriptional factor (MITF), showed light-induced transcription, suggesting a dominating role of morphological color change (MCC) in this process. Transcription of genes related to growth factor, MAPK and PI3K-Akt pathways increased with time of light exposure, suggesting that light could induce significant growth signal, which might facilitate the rapid skin darkening. Most importantly, an in-frame deletion of four residues was identified in O. rhodostigmatus melanocortin-1 receptor (MC1R), a critical receptor in MCC. This deletion results in a more negatively charged ligand pocket with three stereo-tandem aspartate residues. Such structural changes likely decrease the constitutive activity of MC1R, but increase its ligands-dependent activity, thus coordinating pigment regression and rapid melanogenesis in the dark and light, respectively. Conclusion: Our study suggested that rapid MCC was responsible for the light-induced rapid darkening of O. rhodostigmatus tadpoles. Genetic mutations of MC1R in them could explain how these non-obligate cave dwellers coordinate pigment regression and robust melanogenesis in darkness and light, respectively. To our knowledge, this is the first study that reports the association between pigmentation phenotype adaptation and MC1R mutations in amphibians and/or in non-obligate cave dwellers. |
| URL标识 | 查看原文 |
| WOS关键词 | COAT COLOR ; MELANOCORTIN-1 RECEPTOR ; BACKGROUND ADAPTATION ; XENOPUS-LAEVIS ; FEATHER COLOR ; PLUMAGE COLOR ; EVOLUTION ; MITF ; MC1R ; MELANOBLAST |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000433580800002 |
| 出版者 | BIOMED CENTRAL LTD |
| 源URL | [http://210.75.237.14/handle/351003/30247]  |
| 专题 | 食品安全与环境治理领域_中国科学院环境与应用微生物重点实验室 中国科学院成都生物研究所 生物多样性与生态系统服务领域_中国科学院山地生态恢复与生物资源利用重点实验室 生物多样性与生态系统服务领域_生态系统恢复与生物多样性保育四川省重点实验室 |
| 作者单位 | 1.CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Wei,Liu, Lusha,Wang, Xungang,et al. Transcriptomics reveals the molecular processes of light-induced rapid darkening of the non-obligate cave dweller Oreolalax rhodostigmatus (Megophryidae, Anura) and their genetic basis of pigmentation strategy[J]. BMC GENOMICS,2019,19:422. |
| APA | Zhu, Wei,Liu, Lusha,Wang, Xungang,Gao, Xinyu,Jiang, Jianping,&Wang, Bin.(2019).Transcriptomics reveals the molecular processes of light-induced rapid darkening of the non-obligate cave dweller Oreolalax rhodostigmatus (Megophryidae, Anura) and their genetic basis of pigmentation strategy.BMC GENOMICS,19,422. |
| MLA | Zhu, Wei,et al."Transcriptomics reveals the molecular processes of light-induced rapid darkening of the non-obligate cave dweller Oreolalax rhodostigmatus (Megophryidae, Anura) and their genetic basis of pigmentation strategy".BMC GENOMICS 19(2019):422. |
入库方式： OAI收割
来源：成都生物研究所
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