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Characterization of two styrene monooxygenases from marine microbes

文献类型：期刊论文

作者Pu, Wei1,2,3; Cui, Can1,2,3; Guo, Chao2,3; Wu, Zhong-Liu2,3
刊名ENZYME AND MICROBIAL TECHNOLOGY
出版日期2018-05-01
卷号112页码:29-34
关键词Monooxygenase Oxide Whole-cell biocatalyst Epoxidation Asymmetric synthesis
ISSN号0141-0229
DOI10.1016/j.enzmictec.2018.02.001
产权排序1
文献子类Article
英文摘要Styrene monooxygenases (SMOs) are highly stereoselective enzymes that catalyze the formation of chiral epoxides as versatile building blocks. To expand the enzyme toolbox, two bacterial SMOs were identified from the genome of marine microbes Paraglaciecola agarilytica NO2 and Marinobacteriwn litorale DSM 23545, and heterologously expressed in Escherichia coli in soluble form. Both of the resulting whole-cell biocatalysts exhibited maximal activity at 30 degrees C and pH 8.0. They catalyzed the sulfoxidation reactions, and the epoxidation of both conjugated and unconjugated styrene derivatives with up to > 99%ee. MISMO displayed higher activity toward most substrates tested. Compared to an established SMO from Pseudomonas species (PsSMO), MISMO achieved 3.0-, 3.4- and 2.6-fold conversions for substrates styrene, cinnamyl alcohol and 4-vinyl-2, 3-dihydrobenzofuran, respectively.
学科主题Biotechnology ; Applied Microbiology
URL标识查看原文
WOS关键词(S)-STYRENE OXIDE ; ASYMMETRIC EPOXIDATION ; FUNCTIONAL-ANALYSIS ; GENUS GLACIECOLA ; EPOXY ALCOHOLS ; STRAIN VLB120 ; BIOCATALYSIS ; DEGRADATION ; BINDING ; SCALE
WOS研究方向Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:000428099200005
出版者ELSEVIER SCIENCE INC
源URL[http://210.75.237.14/handle/351003/30267]  
专题环境治理与食品安全领域_应用与环境微生物研究
成都生物研究所_应用与环境微生物研究
环境治理与食品安全领域_环境微生物四川省重点实验室
环境治理与食品安全领域_中国科学院环境与应用微生物重点实验室
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Environm Microbiol Key Lab Sichuan Prov, Chengdu 610041, Sichuan, Peoples R China;
3.Chinese Acad Sci, Chengdu Inst Biol, Key Lab Environm & Appl Microbiol, Chengdu 610041, Sichuan, Peoples R China;
推荐引用方式
GB/T 7714		 Pu, Wei,Cui, Can,Guo, Chao,et al. Characterization of two styrene monooxygenases from marine microbes[J]. ENZYME AND MICROBIAL TECHNOLOGY,2018,112:29-34.
APA Pu, Wei,Cui, Can,Guo, Chao,&Wu, Zhong-Liu.(2018).Characterization of two styrene monooxygenases from marine microbes.ENZYME AND MICROBIAL TECHNOLOGY,112,29-34.
MLA Pu, Wei,et al."Characterization of two styrene monooxygenases from marine microbes".ENZYME AND MICROBIAL TECHNOLOGY 112(2018):29-34.

入库方式： OAI收割

来源：成都生物研究所

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