Transcriptome Analysis Provides Insights into Gingerol Biosynthesis in Ginger (Zingiber officinale)
文献类型：期刊论文
| 作者 | Jiang, Yusong2,4; Huang, Mengjun2; Wisniewski, Michael4; Li, Honglei2; Zhang, Meixia2; Tao, Xiang3; Liu, Yiqing2,4; Zou, Yong2 |
| 刊名 | PLANT GENOME
 |
| 出版日期 | 2018-11-01 |
| 卷号 | 11期号:3页码:UNSP 180034 |
| ISSN号 | 1940-3372 |
| DOI | 10.3835/plantgenome2018.06.0034 |
| 产权排序 | 4 |
| 文献子类 | Article |
| 英文摘要 | Ginger (Zingiber officinale Roscoe), a perennial herb, is one of the most economically valuable plants in the Zingiberaceae family. Gingerol, as the major constituents of ginger essential oil, contributes to the unique flavor and pharmaceutical value of ginger. However, the pathway of gingerol biosynthesis has not been verified and described in ginger to help understand the biosynthesis of secondary metabolites in nonmodel species. In this study, the concentrations of gingerols were quantified at different stages of rhizome development and in different tissues. The results confirmed that rhizomes are the major source of gingerols and that accumulation of gingerols in the rhizome starts at an early developmental stage. We also assembled a reference ginger transcriptome, which is composed of 219,479 unigenes consisting of 330,568 transcripts and provides a high-quality genetic resource for further research. An analysis of differentially expressed genes (DEGs) identified 12,935 DEGs among several different comparisons. Five genes [ curcumin synthase (CURS), cinnamate 4-hydroxylase (CYP73A), p-coumaroyl quinate/shikimate 3'-hydroxylase (CYP98A), caffeoyl-coenzyme A O-methyl transferase (CCoAOMT), and hydroxycinnamoyl-coenzyme A shikimate/quinate hydroxycinnamoyl transferase (HCT)] associated with gingerol biosynthesis were identified as being significantly differentially expressed in the rhizome at an early developmental stage and all five genes were upregulated. Expression analysis revealed that different loci of these genes have become functionally specialized in different tissues and different developmental stages of the rhizome (subfunctionalization). Among the DEGs, CCoAOMT and HCT may act as gatekeepers and rate-limiting enzymes in the gingerol biosynthesis pathway and thus play an important role in regulating the biosynthesis of gingerol. |
| 学科主题 | Plant Sciences |
| URL标识 | 查看原文 |
| WOS关键词 | EXPRESSION ANALYSIS ; GENE ONTOLOGY ; 6-GINGEROL ; ANNOTATION ; APOPTOSIS ; ANTIOXIDANT ; PLATFORM ; COLITIS ; GENOME ; MICE |
| WOS研究方向 | Plant Sciences ; Genetics & Heredity |
| 语种 | 英语 |
| 出版者 | CROP SCIENCE SOC AMER |
| WOS记录号 | WOS:000450930200016 |
| 源URL | [http://210.75.237.14/handle/351003/30120]  |
| 专题 | 环境治理与食品安全领域_应用与环境微生物研究 环境治理与食品安全领域_农业生物技术研究 |
| 作者单位 | 1.USDA ARS, Kearneysville, WV 25430 USA; 2.Chongqing Univ Arts & Sci, Res Inst Special Plants, Chongqing 402160, Peoples R China; 3.Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China 4.Chongqing Key Lab Econ Plant Biotechnol, Chongqing 402168, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Jiang, Yusong,Huang, Mengjun,Wisniewski, Michael,et al. Transcriptome Analysis Provides Insights into Gingerol Biosynthesis in Ginger (Zingiber officinale)[J]. PLANT GENOME,2018,11(3):UNSP 180034. |
| APA | Jiang, Yusong.,Huang, Mengjun.,Wisniewski, Michael.,Li, Honglei.,Zhang, Meixia.,...&Zou, Yong.(2018).Transcriptome Analysis Provides Insights into Gingerol Biosynthesis in Ginger (Zingiber officinale).PLANT GENOME,11(3),UNSP 180034. |
| MLA | Jiang, Yusong,et al."Transcriptome Analysis Provides Insights into Gingerol Biosynthesis in Ginger (Zingiber officinale)".PLANT GENOME 11.3(2018):UNSP 180034. |
入库方式： OAI收割
来源：成都生物研究所
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