Frond transformation system mediated by Agrobacterium tumefaciens for Lemna minor
文献类型：期刊论文
| 作者 | Yang, Gui-Li1,2,3; Fang, Yang1,3; Xu, Ya-Liang1,2,3; Tan, Li1,3; Li, Qi1,2,3; Liu, Yang1,2,3; Lai, Fan1,2,3; Jin, Yan-Ling1,3; Du, An-Ping1,3; He, Kai-Ze1,3 |
| 刊名 | PLANT MOLECULAR BIOLOGY
 |
| 出版日期 | 2018-11-01 |
| 卷号 | 98期号:2018页码:319-331 |
| 关键词 | Duckweed Lemna minor Frond transformation system Agrobacterium tumefaciens Plant bioreactor Stigmasterol |
| ISSN号 | 0167-4412 |
| DOI | 10.1007/s11103-018-0778-x |
| 产权排序 | 1 |
| 文献子类 | Article |
| 英文摘要 | The Lemnaceae, known as duckweed, the smallest flowering aquatic plant, shows promise as a plant bioreactor. For applying this potential plant bioreactor, establishing a stable and efficient genetic transformation system is necessary. The currently favored callus-based method for duckweed transformation is time consuming and genotype limited, as it requires callus culture and regeneration, which is inapplicable to many elite duckweed strains suitable for bioreactor exploitation. In this study, we attempted to establish a simple frond transformation system mediated by Agrobacterium tumefaciens for Lemna minor, one of the most widespread duckweed species in the world. To evaluate the feasibility of the new transformation system, the gene CYP710A11 was overexpressed to improve the yield of stigmasterol, which has multiple medicinal purposes. Three L. minor strains, ZH0055, D0158 and M0165, were transformed by both a conventional callus transformation system (CTS) and the simple frond transformation system (FTS). GUS staining, PCR, quantitative PCR and stigmasterol content detection showed that FTS can produce stable transgenic lines as well as CTS. Moreover, compared to CTS, FTS can avoid the genotype constraints of callus induction, thus saving at least half of the required processing time (CTS took 8-9 months while FTS took approximately 3 months in this study). Therefore, this transformation system is feasible in producing stable transgenic lines for a wide range of L. minor genotypes. |
| 学科主题 | Plant Sciences |
| URL标识 | 查看原文 |
| WOS关键词 | DISCHARGE PARTICLE-ACCELERATION ; CALLUS INDUCTION ; WASTE-WATER ; BIOMASS PRODUCTION ; DUCKWEED ; EXPRESSION ; STIGMASTEROL ; PLANTS ; REGENERATION ; GENE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000450658300003 |
| 出版者 | SPRINGER |
| 源URL | [http://210.75.237.14/handle/351003/30121]  |
| 专题 | 环境治理与食品安全领域_应用与环境微生物研究 环境治理与食品安全领域_环境微生物四川省重点实验室 环境治理与食品安全领域_中国科学院环境与应用微生物重点实验室 |
| 作者单位 | 1.Environm Microbiol Key Lab Sichuan Prov, Chengdu 610041, Sichuan, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Chengdu Inst Biol, Key Lab Environm & Appl Microbiol, Chengdu 610041, Sichuan, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Yang, Gui-Li,Fang, Yang,Xu, Ya-Liang,et al. Frond transformation system mediated by Agrobacterium tumefaciens for Lemna minor[J]. PLANT MOLECULAR BIOLOGY,2018,98(2018):319-331. |
| APA | Yang, Gui-Li.,Fang, Yang.,Xu, Ya-Liang.,Tan, Li.,Li, Qi.,...&Zhao, Hai.(2018).Frond transformation system mediated by Agrobacterium tumefaciens for Lemna minor.PLANT MOLECULAR BIOLOGY,98(2018),319-331. |
| MLA | Yang, Gui-Li,et al."Frond transformation system mediated by Agrobacterium tumefaciens for Lemna minor".PLANT MOLECULAR BIOLOGY 98.2018(2018):319-331. |
入库方式： OAI收割
来源：成都生物研究所
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