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Cys(2)His(2) Zinc Finger Transcription Factor BcabaR1 Positively Regulates Abscisic Acid Production in Botrytis cinerea

文献类型：期刊论文

作者Wang, Yingming1,2,3; Zhou, Jinyan2,3; Zhong, Juan2,3; Luo, Di2,3; Li, Zhemin2,3; Yang, Jie2,3; Shu, Dan2,3; Tan, Hong2,3
刊名APPLIED AND ENVIRONMENTAL MICROBIOLOGY
出版日期2018-09-01
卷号84期号:17页码:UNSP e00920-18
关键词abscisic acid transcription factor promoter Botrytis cinerea
ISSN号0099-2240
DOI10.1128/AEM.00920-18
产权排序1
文献子类Article
英文摘要Abscisic acid (ABA) is one of the five classical phytohormones involved in increasing the tolerance of plants for various kinds of stresses caused by abiotic or biotic factors, and it also plays important roles in regulating the activation of innate immune cells and glucose homeostasis in mammals. For these reasons, as a "stress hormone," ABA has recently received attention as a candidate drug for agriculture and biomedical applications, prompting significant development of ABA synthesis. Some plant-pathogenic fungi can synthesize natural ABA. The fungus Botrytis cinerea has been used for biotechnological production of ABA. Identification of the transcription factors (TFs) involved in regulation of ABA biosynthesis in B. cinerea would provide new clues to understand how ABA is synthesized and regulated. In this study, we defined a novel Cys(2)His(2) TF, BcabaRl, that regulates the transcriptional levels of ABA synthase genes (bcaba1, bcaba2, bcaba3, and bcaba4) in an ABA-overproducing mutant, B. cinerea TBC-A. Electrophoretic mobility shift assays revealed that recombinant BcabaR1 can bind specifically to both a 14-nucleotide sequence motif and a 39-nucleotide sequence motif in the promoter region of bcaba1 to -4 genes in vitro. A decreased transcriptional level of the bcabaR1 gene in B. cinerea led to significantly decreased ABA production and downregulated transcription of bcaba1 to -4. When bcabaR1 was overexpressed in B. cinerea, ABA production was significantly increased, with upregulated transcription of bcaba1 to -4. Thus, in this study, we found that BcabaR1 acts as a positive regulator of ABA biosynthesis in B. cinerea. IMPORTANCE Abscisic acid (ABA) could make a potentially important contribution to theoretical research and applications in agriculture and medicine. Botrytis cinerea is a plant-pathogenic fungus that was found to produce ABA. There has been a view that ABA is related to the interaction between pathogenic fungi and plants. Identification of regulatory genes involved in ABA biosynthesis may facilitate an understanding of the underlying molecular mechanisms of ABA biosynthesis and the pathogenesis of B. cinerea. Here, we present a positive regulator, BcabaR1, of ABA biosynthesis in B. cinerea that can affect the transcriptional level of the ABA biosynthesis gene cluster, bcaba1 to -4, by directly binding to the conserved sequence elements in the promoter of the bcaba1 to -4 genes. This TF was found to be specifically involved in regulation of ABA biosynthesis. This work provides new clues for finding other ABA biosynthesis genes and improving ABA yield in B. cinerea.
学科主题Biology
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WOS关键词CYCLIC ADP-RIBOSE ; GENE-CLUSTER ; SECONDARY METABOLISM ; CERCOSPORA-ROSICOLA ; IDENTIFICATION ; BIOSYNTHESIS ; REVEALS ; FUNGI ; MANIPULATION ; PHOSPHATASE
WOS研究方向Biotechnology & Applied Microbiology ; Microbiology
语种英语
WOS记录号WOS:000441894000009
出版者AMER SOC MICROBIOLOGY
源URL[http://210.75.237.14/handle/351003/30171]  
专题环境治理与食品安全领域_应用与环境微生物研究
环境治理与食品安全领域_中国科学院环境与应用微生物重点实验室
作者单位1.Univ Chinese Acad Sci, Beijing, Peoples R China
2.Chinese Acad Sci, Chengdu Inst Biol, Key Lab Environm & Appl Microbiol, Chengdu, Sichuan, Peoples R China;
3.Environm Microbiol Key Lab Sichuan Prov, Chengdu, Sichuan, Peoples R China;
推荐引用方式
GB/T 7714		 Wang, Yingming,Zhou, Jinyan,Zhong, Juan,et al. Cys(2)His(2) Zinc Finger Transcription Factor BcabaR1 Positively Regulates Abscisic Acid Production in Botrytis cinerea[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2018,84(17):UNSP e00920-18.
APA Wang, Yingming.,Zhou, Jinyan.,Zhong, Juan.,Luo, Di.,Li, Zhemin.,...&Tan, Hong.(2018).Cys(2)His(2) Zinc Finger Transcription Factor BcabaR1 Positively Regulates Abscisic Acid Production in Botrytis cinerea.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,84(17),UNSP e00920-18.
MLA Wang, Yingming,et al."Cys(2)His(2) Zinc Finger Transcription Factor BcabaR1 Positively Regulates Abscisic Acid Production in Botrytis cinerea".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 84.17(2018):UNSP e00920-18.

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来源：成都生物研究所

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