Structural Insight into the Allosteric Coupling of Cu1 Site and Trinuclear Cu Cluster in CotA Laccase
文献类型：期刊论文
| 作者 | Xie, Tian1,2; Liu, Zhongchuan1,2; Wang, Ganggang1,2 |
| 刊名 | CHEMBIOCHEM
 |
| 出版日期 | 2019-07-16 |
| 卷号 | 19期号:14页码:1502-1506 |
| 关键词 | allosterism copper enzymes hydrogen bonds structure elucidation |
| ISSN号 | 1439-4227 |
| DOI | 10.1002/cbic.201800236 |
| 产权排序 | 1 |
| 文献子类 | Article |
| 英文摘要 | In laccase, type1 copper (Cu1) was connected to the trinuclear copper center (TNC) by the conserved Cys-His bridge. An allosteric coupling between the two redox sites has been reported; however, the molecular mechanism underlining the allosteric coupling is unknown. In this study, ligands of the two type3 copper sites, including His491 and His493, in CotA were mutated to Cys or Ala. The crystal structures revealed that mutations at His491 and His493 caused rearrangement of the hydrogen-bond network and geometric distortion of the TNC, which severely impaired the activities of mutants H493A, H493C, and H491C. In addition, the change in TNC affected hydrogen bonds around Cys492 in the mutants and led to Cu1 being partially reduced. These results not only decipher the mechanism of allosteric coupling between Cu1 and TNC in laccase, but also pave the way for laccase protein engineering. |
| 学科主题 | Chemistry ; Analysis |
| URL标识 | 查看原文 |
| WOS关键词 | MULTICOPPER OXIDASE FET3P ; CRYSTAL-STRUCTURE ; SUBSTRATE ; OXIDATION ; MUTANTS ; O-2 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000439494900008 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://210.75.237.14/handle/351003/30204]  |
| 专题 | 环境治理与食品安全领域_应用与环境微生物研究 环境治理与食品安全领域_环境微生物四川省重点实验室 环境治理与食品安全领域_中国科学院环境与应用微生物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Environm Microbiol Sichuan Prov, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China 2.Chinese Acad Sci, Chengdu Inst Biol, Key Lab Environm & Appl Microbiol, Chengdu 610041, Sichuan, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Xie, Tian,Liu, Zhongchuan,Wang, Ganggang. Structural Insight into the Allosteric Coupling of Cu1 Site and Trinuclear Cu Cluster in CotA Laccase[J]. CHEMBIOCHEM,2019,19(14):1502-1506. |
| APA | Xie, Tian,Liu, Zhongchuan,&Wang, Ganggang.(2019).Structural Insight into the Allosteric Coupling of Cu1 Site and Trinuclear Cu Cluster in CotA Laccase.CHEMBIOCHEM,19(14),1502-1506. |
| MLA | Xie, Tian,et al."Structural Insight into the Allosteric Coupling of Cu1 Site and Trinuclear Cu Cluster in CotA Laccase".CHEMBIOCHEM 19.14(2019):1502-1506. |
入库方式： OAI收割
来源：成都生物研究所
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