Solution scattering study of the Bacillus subtilis PgdS enzyme involved in poly-gamma-glutamic acids degradation
文献类型：期刊论文
| 作者 | Zeng, Jumei; Jin, Yun; Liu, Zhongchuan |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2019-04-02 |
| 卷号 | 13期号:4页码:e195355 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0195355 |
| 产权排序 | 1 |
| 文献子类 | Article |
| 英文摘要 | The PgdS enzyme is a poly-gamma-glutamic (gamma-PGA) hydrolase, which has potential application for a controllable degradation of gamma-PGA by enzymatic depolymerization; however, the structure of PgdS is still unknown. Here, to study in detail the full-length PgdS structure, we analyze the low-resolution architecture of PgdS hydrolase from Bacillus subtilis in solution using small angle X-ray scattering (SAXS) method. Combining with other methods, like dynamic light scattering and mutagenesis analyses, a model for the full length structure and the possible substrate delivery route of PgdS are proposed. The results will provide useful hints for future investigations into the mechanisms of gamma-PGA degradation by the PgdS hydrolase and may provide valuable practical information. |
| URL标识 | 查看原文 |
| WOS关键词 | SMALL-ANGLE SCATTERING ; SEQUENCE ALIGNMENT ; POLYGLUTAMIC ACID ; ENDOPEPTIDASE ; PROTEIN ; RECOGNITION ; PRODUCT ; GENE ; LYTF |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000428988800048 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 源URL | [http://210.75.237.14/handle/351003/30281]  |
| 专题 | 环境治理与食品安全领域_应用与环境微生物研究 环境治理与食品安全领域_中国科学院环境与应用微生物重点实验室 |
| 作者单位 | Chinese Acad Sci, Chengdu Inst Biol, Key Lab Environm & Appl Microbiol, Chengdu, Sichuan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zeng, Jumei,Jin, Yun,Liu, Zhongchuan. Solution scattering study of the Bacillus subtilis PgdS enzyme involved in poly-gamma-glutamic acids degradation[J]. PLOS ONE,2019,13(4):e195355. |
| APA | Zeng, Jumei,Jin, Yun,&Liu, Zhongchuan.(2019).Solution scattering study of the Bacillus subtilis PgdS enzyme involved in poly-gamma-glutamic acids degradation.PLOS ONE,13(4),e195355. |
| MLA | Zeng, Jumei,et al."Solution scattering study of the Bacillus subtilis PgdS enzyme involved in poly-gamma-glutamic acids degradation".PLOS ONE 13.4(2019):e195355. |
入库方式： OAI收割
来源：成都生物研究所
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