New Insights into the Function and Global Distribution of Polyethylene Terephthalate (PET)-Degrading Bacteria and Enzymes in Marine and Terrestrial Metagenomes
文献类型：期刊论文
| 作者 | Danso, Dominik1; Schmeisser, Christel1; Chow, Jennifer1; Zimmermann, Wolfgang5; Wei, Ren5; Leggewie, Christian4; Li, Xiangzhen3; Hazen, Terry2; Streit, Wolfgang R.1 |
| 刊名 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY
 |
| 出版日期 | 2018-04-01 |
| 卷号 | 84期号:8页码:UNSP e02773-17 |
| 关键词 | HMM hydrolases metagenome metagenomic screening PET degradation polyethylene terephthalate (PET) BHET TPA metagenomes |
| ISSN号 | 0099-2240 |
| DOI | 10.1128/AEM.02773-17 |
| 产权排序 | 4 |
| 文献子类 | Article |
| 英文摘要 | Polyethylene terephthalate (PET) is one of the most important synthetic polymers used today. Unfortunately, the polymers accumulate in nature and to date no highly active enzymes are known that can degrade it at high velocity. Enzymes involved in PET degradation are mainly alpha- and beta-hydrolases, like cutinases and related enzymes (EC 3.1.1). Currently, only a small number of such enzymes are well characterized. In this work, a search algorithm was developed that identified 504 possible PET hydrolase candidate genes from various databases. A further global search that comprised more than 16 Gb of sequence information within 108 marine and 25 terrestrial metagenomes obtained from the Integrated Microbial Genome (IMG) database detected 349 putative PET hydrolases. Heterologous expression of four such candidate enzymes verified the function of these enzymes and confirmed the usefulness of the developed search algorithm. In this way, two novel and thermostable enzymes with high potential for downstream application were partially characterized. Clustering of 504 novel enzyme candidates based on amino acid similarities indicated that PET hydrolases mainly occur in the phyla of Actinobacteria, Proteobacteria, and Bacteroidetes. Within the Proteobacteria, the Betaproteobacteria, Deltaproteobacteria, and Gammaproteobacteria were the main hosts. Remarkably enough, in the marine environment, bacteria affiliated with the phylum Bacteroidetes appear to be the main hosts of PET hydrolase genes, rather than Actinobacteria or Proteobacteria, as observed for the terrestrial metagenomes. Our data further imply that PET hydrolases are truly rare enzymes. The highest occurrence of 1.5 hits/Mb was observed in sequences from a sample site containing crude oil. IMPORTANCE Polyethylene terephthalate (PET) accumulates in our environment without significant microbial conversion. Although a few PET hydrolases are already known, it is still unknown how frequently they appear and with which main bacterial phyla they are affiliated. In this study, deep sequence mining of protein databases and metagenomes demonstrated that PET hydrolases indeed occur at very low frequencies in the environment. Furthermore, it was possible to link them to phyla that were previously not known to harbor such enzymes. This work contributes novel knowledge on the phylogenetic relationships, the recent evolution, and the global distribution of PET hydrolases. Finally, we describe the biochemical traits of four novel PET hydrolases. |
| 学科主题 | Biology |
| URL标识 | 查看原文 |
| WOS关键词 | BIODEGRADATION ; HYDROLASE ; HYDROLYSIS ; DATABASE ; COMPOST ; FUSCA ; PET |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000429665700024 |
| 出版者 | AMER SOC MICROBIOLOGY |
| 源URL | [http://210.75.237.14/handle/351003/30282]  |
| 专题 | 环境治理与食品安全领域_应用与环境微生物研究 |
| 作者单位 | 1.Univ Hamburg, Dept Microbiol & Biotechnol, Bioctr Klein Flottbek, Hamburg, Germany; 2.Univ Tennessee, Knoxville, TN USA 3.Chengdu Inst Biol, Chengdu, Sichuan, Peoples R China; 4.Evoxx Technol GmbH, Monheim, Germany; 5.Univ Leipzig Univ, Dept Microbiol & Bioproc Technol, Inst Biochem, Leipzig, Germany; |
| 推荐引用方式 GB/T 7714 | Danso, Dominik,Schmeisser, Christel,Chow, Jennifer,et al. New Insights into the Function and Global Distribution of Polyethylene Terephthalate (PET)-Degrading Bacteria and Enzymes in Marine and Terrestrial Metagenomes[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2018,84(8):UNSP e02773-17. |
| APA | Danso, Dominik.,Schmeisser, Christel.,Chow, Jennifer.,Zimmermann, Wolfgang.,Wei, Ren.,...&Streit, Wolfgang R..(2018).New Insights into the Function and Global Distribution of Polyethylene Terephthalate (PET)-Degrading Bacteria and Enzymes in Marine and Terrestrial Metagenomes.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,84(8),UNSP e02773-17. |
| MLA | Danso, Dominik,et al."New Insights into the Function and Global Distribution of Polyethylene Terephthalate (PET)-Degrading Bacteria and Enzymes in Marine and Terrestrial Metagenomes".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 84.8(2018):UNSP e02773-17. |
入库方式： OAI收割
来源：成都生物研究所
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