Simultaneous quantitative assessment of nine glycosides in tobacco by liquid chromatography-tandem mass spectrometry
文献类型：期刊论文
| 作者 | Yuan, Yue1; Zhou, Rong1; Li, Dongliang1; Luo, Cheng1; Li, Guoyou2 |
| 刊名 | JOURNAL OF SEPARATION SCIENCE
 |
| 出版日期 | 2018-03-01 |
| 卷号 | 41期号:5页码:1009-1016 |
| 关键词 | endogenous glycosides response surface methodology simultaneous quantification tobacco ultra high performance liquid chromatography |
| ISSN号 | 1615-9306 |
| DOI | 10.1002/jssc.201700880 |
| 产权排序 | 2 |
| 文献子类 | Article |
| 英文摘要 | A simple and efficient method combining ultrasound-assisted extraction, the conditions of which were optimized by response surface methodology, with liquid chromatography and tandem mass spectrometry was established and validated for the absolute quantification of nine non-volatile neutral glycosides originating from tobacco (Nicotiana tobaccum L.) leaves, comprising three phenolic glycosides, one benzanoid glycoside, and five sesquiterpene glycosides within three isomers, originating from tobacco leaves. Factors of extraction time, sample quantity, extraction solvent, liquid chromatographic conditions, and electrospray ionization parameters were carefully investigated to ensure the selectivity and sensitivity of the method. All calibration curves showed excellent coefficients of determination ranging from 0.9940 to 0.9996, within the range of tested concentrations. The limits of detection and quantification were 2.33-25.9 and 7.06-78.5 ng/mL, respectively. Satisfactory values of accuracy were between 80.1 to 107.9% among different sample matrixes. The relative standard deviations of intra-and inter-day analysis were less than 13.7 and 13.0% respectively. The developed method was successfully applied in a pilot study to determine the amounts of the nine endogenous glycosides in real flue-cured tobacco samples obtained from different habitats in China. |
| 学科主题 | Spectroscopy ; Instrumentation ; Analytical Sciences |
| URL标识 | 查看原文 |
| WOS关键词 | RESPONSE-SURFACE METHODOLOGY ; GAS-CHROMATOGRAPHY ; SIMULTANEOUS DISTILLATION ; NONVOLATILE CONSTITUENTS ; SOLVENT-EXTRACTION ; LEAVES ; IDENTIFICATION ; GLUCOSIDES ; MS ; OPTIMIZATION |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000426734800002 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://210.75.237.14/handle/351003/30316]  |
| 专题 | 环境治理与食品安全领域_应用与环境微生物研究 |
| 作者单位 | 1.China Tobacco Sichuan Ind Co Ltd, Tech Res Ctr, Chengdu 610066, Sichuan, Peoples R China; 2.Chinese Acad Sci, Chengdu Inst Biol, Chengdu, Sichuan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yuan, Yue,Zhou, Rong,Li, Dongliang,et al. Simultaneous quantitative assessment of nine glycosides in tobacco by liquid chromatography-tandem mass spectrometry[J]. JOURNAL OF SEPARATION SCIENCE,2018,41(5):1009-1016. |
| APA | Yuan, Yue,Zhou, Rong,Li, Dongliang,Luo, Cheng,&Li, Guoyou.(2018).Simultaneous quantitative assessment of nine glycosides in tobacco by liquid chromatography-tandem mass spectrometry.JOURNAL OF SEPARATION SCIENCE,41(5),1009-1016. |
| MLA | Yuan, Yue,et al."Simultaneous quantitative assessment of nine glycosides in tobacco by liquid chromatography-tandem mass spectrometry".JOURNAL OF SEPARATION SCIENCE 41.5(2018):1009-1016. |
入库方式： OAI收割
来源：成都生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。