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Crystal structure of IspF from Bacillus subtilis and absence of protein complex assembly amongst IspD/IspE/IspF enzymes in the MEP pathway

文献类型：期刊论文

作者Liu, Zhongchuan1,2; Jin, Yun1,2,4; Liu, Weifeng3; Tao, Yong3; Wang, Ganggang1,2
刊名BIOSCIENCE REPORTS
出版日期2019-02-28
卷号38页码:BSR20171370
ISSN号0144-8463
DOI10.1042/BSR20171370
产权排序1
文献子类Article
英文摘要2-C-Methyl-D-erythritol 2,4-cyclodiphosphate synthase (IspF) is a key enzyme in the 2-C-Methyl-D-erythritol-4-phosphate (MEP) pathway of isoprenoid biosynthesis. This enzyme catalyzes the 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDPME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MEcDP) with concomitant release of cytidine 5'-diphospate (CMP). Bacillus subtilis is a potential host cell for the production of isoprenoids, but few studies are performed on the key enzymes of MEP pathway in B. subtilis. In this work, the high-resolution crystal structures of IspF in native and complex with CMP from B. subtilis have been determined. Structural comparisons indicate that there is a looser packing of the subunits of IspF in B. subtilis, whereas the solvent accessible surface of its active pockets is smaller than that in Escherichia coli. Meanwhile, the protein-protein associations of 2-C-Methyl-D-erythritol-4-phosphatecytidyltransferase (IspD), CDPME kinase (IspE) and IspF from B. subtilis and E. coli, which catalyze three consecutive steps in the MEP pathway, are analyzed by native gel shift and size exclusion chromatography methods. The data here show that protein complex assembly is not detectable. These results will be useful for isoprenoid biosynthesis by metabolic engineering.
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WOS关键词NON-MEVALONATE PATHWAY ; COLI 2C-METHYL-D-ERYTHRITOL-2,4-CYCLODIPHOSPHATE SYNTHASE ; METHYLERYTHRITOL PHOSPHATE-PATHWAY ; ISOPRENOID PRECURSOR PATHWAY ; 2,4-CYCLODIPHOSPHATE SYNTHASE ; ARABIDOPSIS-THALIANA ; SEQUENCE ALIGNMENT ; BIOSYNTHESIS ; INHIBITORS ; BACTERIA
WOS研究方向Biochemistry & Molecular Biology ; Cell Biology
语种英语
WOS记录号WOS:000462677200004
出版者PORTLAND PRESS LTD
源URL[http://210.75.237.14/handle/351003/30327]  
专题环境治理与食品安全领域_应用与环境微生物研究
环境治理与食品安全领域_环境微生物四川省重点实验室
环境治理与食品安全领域_中国科学院环境与应用微生物重点实验室
作者单位1.Chinese Acad Sci, Chengdu Inst Biol, Key Lab Environm & Appl Microbiol, Chengdu 610041, Sichuan, Peoples R China;
2.Key Lab Environm Microbiol Sichuan Prov, Chengdu 610041, Sichuan, Peoples R China;
3.Chinese Acad Sci, Inst Microbiol, Key Lab Microbial Physiol & Metab Engn, Beijing 100101, Peoples R China;
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Liu, Zhongchuan,Jin, Yun,Liu, Weifeng,et al. Crystal structure of IspF from Bacillus subtilis and absence of protein complex assembly amongst IspD/IspE/IspF enzymes in the MEP pathway[J]. BIOSCIENCE REPORTS,2019,38:BSR20171370.
APA Liu, Zhongchuan,Jin, Yun,Liu, Weifeng,Tao, Yong,&Wang, Ganggang.(2019).Crystal structure of IspF from Bacillus subtilis and absence of protein complex assembly amongst IspD/IspE/IspF enzymes in the MEP pathway.BIOSCIENCE REPORTS,38,BSR20171370.
MLA Liu, Zhongchuan,et al."Crystal structure of IspF from Bacillus subtilis and absence of protein complex assembly amongst IspD/IspE/IspF enzymes in the MEP pathway".BIOSCIENCE REPORTS 38(2019):BSR20171370.

入库方式： OAI收割

来源：成都生物研究所

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