Discriminating five Polygonatum medical materials and monitoring their chemical changes associated with traditional process by FT-IR spectroscopy coupled with multivariate analysis
文献类型：期刊论文
| 作者 | Luo, Congpei1,2; Li, Wei2; Chun, Ze2; Wu, Yu2 |
| 刊名 | VIBRATIONAL SPECTROSCOPY
 |
| 出版日期 | 2018-11-01 |
| 卷号 | 99期号:2018页码:104-112 |
| 关键词 | Polygonatum Discrimination Quality control Fourier transform infrared spectroscopy Multivariate analysis Traditional process |
| ISSN号 | 0924-2031 |
| DOI | 10.1016/j.vibspec.2018.08.016 |
| 产权排序 | 1 |
| 文献子类 | Article |
| 英文摘要 | Several Polygonatwn species are important medicinal materials as tonic to cure disorders in China. Because of their different medical effects, it is desired to distinguish them at species level. In addition, to ensure and control their medical quality, it is also important to monitor their chemical changes associated with traditional process. Taking the advantages of Fourier transform infrared spectroscopy (FT-IR) and multivariate analysis, we developed a convenient, fast and reliable approach to discriminate and quality control these materials. Despite similar absorption patterns, each species also presented spectral differences, especially on the FT-IR fingerprint range of 1800-600 cm(-1). Second derivative method obviously enlarged those differences and then showed more species specific features. These spectral differences could be used as powerful discriminating points to distinguish them. PCA results showed that each species separated clearly with their biological replicates grouped together, which indicated that the variance between species is greater than within species, therefore, these species could be distinguishable. Using this approach, the five herbal materials were discriminated successfully in their raw, processed and ethanol extracted formats. On the other hand, visual inspecting infrared spectra of samples from 1 to 9 process steps, absorbance near 1737, 1259, 817 and 780 cm(-1) increased gradually but decreased gradually at 927 cm(-1). Besides, spectral contour near 1050 cm(-1) changed sharply with process treatment. These spectral changes indicated that hydrolyzing polysaccharides into oligo-and mono-saccharides, especially glucose and fructose, are the main chemical changes associated with traditional process. This is consistent with the traditional experience that the processed materials are dark as night and sweet as malt sugar. Meanwhile, our results also indicated that their chemical constituents changed profoundly after process, which might be the chemical basis for raw and processed materials have different medical effects. Based on absorbance at 817 and 780 cm(-1) and the color, taste, smell of processed materials followed by energy efficacy, raw materials had to be processed more than 21 h to ensure their quality. This research shows the potential of FT-IR spectroscopy coupled with multivariate analysis to discriminate different herbs and to monitor chemical changes with process and then control their quality. This could be very helpful to ensure the quality, safety, and efficacy of herbs on clinical practices. |
| 学科主题 | Spectroscopy ; Instrumentation ; Analytical Sciences |
| URL标识 | 查看原文 |
| WOS关键词 | INFRARED-SPECTROSCOPY |
| WOS研究方向 | Chemistry ; Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000451494100015 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://210.75.237.14/handle/351003/30126]  |
| 专题 | 环境治理与食品安全领域_农业生物技术研究 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Chengdu Inst Biol, Lab Mt Ecol Restorat & Bioresource Utilizat, Chengdu 610041, Sichuan, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Luo, Congpei,Li, Wei,Chun, Ze,et al. Discriminating five Polygonatum medical materials and monitoring their chemical changes associated with traditional process by FT-IR spectroscopy coupled with multivariate analysis[J]. VIBRATIONAL SPECTROSCOPY,2018,99(2018):104-112. |
| APA | Luo, Congpei,Li, Wei,Chun, Ze,&Wu, Yu.(2018).Discriminating five Polygonatum medical materials and monitoring their chemical changes associated with traditional process by FT-IR spectroscopy coupled with multivariate analysis.VIBRATIONAL SPECTROSCOPY,99(2018),104-112. |
| MLA | Luo, Congpei,et al."Discriminating five Polygonatum medical materials and monitoring their chemical changes associated with traditional process by FT-IR spectroscopy coupled with multivariate analysis".VIBRATIONAL SPECTROSCOPY 99.2018(2018):104-112. |
入库方式： OAI收割
来源：成都生物研究所
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