ROS-Mediated 15-Hydroxyprostaglandin Dehydrogenase Degradation via Cysteine Oxidation Promotes NAD(+)-Mediated Epithelial-Mesenchymal Transition
文献类型：期刊论文
| 作者 | Wang, Weixuan1; Hu, Yadong1,3; Wang, Xiaofei1; Wang, Qingtao2; Deng, Haiteng1 |
| 刊名 | CELL CHEMICAL BIOLOGY
 |
| 出版日期 | 2019-03-15 |
| 卷号 | 25期号:3页码:255-261 |
| ISSN号 | 2451-9448 |
| DOI | 10.1016/j.chembiol.2017.12.008 |
| 产权排序 | 2 |
| 文献子类 | Article |
| 英文摘要 | Nicotinamide adenine dinucleotide (NAD) levels decrease with aging as a result of aging-associated CD38 upregulation. Here, we established a cell model with decreased cellular NAD levels by overexpressing CD38 or treating cells with FK866, an inhibitor of nicotinamide phosphoribosyltransferase. We revealed that decreased NAD triggered reactive oxygen species (ROS)-mediated degradation of 15-hydroxyprostaglandin dehydrogenase (15-PGDH), which drove cells to undergo epithelial-mesenchymal transition (EMT). Moreover, we showed that oxidation of the Cys44 residue to sulfonic acid in 15-PGDH led to its degradation via non-canonical ubiquitination-proteasome and autophagy pathways. Mutation of Cys44 to alanine abolished ROS-induced 15-PGDH degradation. We demonstrated that 15-PGDH silencing promoted EMT, whereas supplementation with NAD precursors increased NAD and 15-PGDH stability, and reversed the EMT process. Taken together, these results suggest that declining NAD levels contribute to age-dependent increases in cancer incidence, and repletion of NAD precursors is beneficial for increasing 15-PGDH expression. |
| 学科主题 | Biochemistry ; Biophysics |
| URL标识 | 查看原文 |
| WOS关键词 | LUNG-CANCER ; IMPROVES MITOCHONDRIAL ; CELLULAR NAD ; LIFE-SPAN ; CD38 ; SUPPRESSOR ; AGE ; METABOLISM ; ACTIVATION ; PROTEINS |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000427600400005 |
| 出版者 | CELL PRESS |
| 源URL | [http://210.75.237.14/handle/351003/30298]  |
| 专题 | 环境治理与食品安全领域_农业生物技术研究 |
| 作者单位 | 1.Tsinghua Univ, Sch Life Sci, MOE Key Lab Bioinformat, Beijing 100084, Peoples R China; 2.Capital Med Univ, Beijing Chaoyang Hosp, Beijing 100043, Peoples R China 3.Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Wang, Weixuan,Hu, Yadong,Wang, Xiaofei,et al. ROS-Mediated 15-Hydroxyprostaglandin Dehydrogenase Degradation via Cysteine Oxidation Promotes NAD(+)-Mediated Epithelial-Mesenchymal Transition[J]. CELL CHEMICAL BIOLOGY,2019,25(3):255-261. |
| APA | Wang, Weixuan,Hu, Yadong,Wang, Xiaofei,Wang, Qingtao,&Deng, Haiteng.(2019).ROS-Mediated 15-Hydroxyprostaglandin Dehydrogenase Degradation via Cysteine Oxidation Promotes NAD(+)-Mediated Epithelial-Mesenchymal Transition.CELL CHEMICAL BIOLOGY,25(3),255-261. |
| MLA | Wang, Weixuan,et al."ROS-Mediated 15-Hydroxyprostaglandin Dehydrogenase Degradation via Cysteine Oxidation Promotes NAD(+)-Mediated Epithelial-Mesenchymal Transition".CELL CHEMICAL BIOLOGY 25.3(2019):255-261. |
入库方式： OAI收割
来源：成都生物研究所
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