Do longer root hairs improve phosphorus uptake? Testing the hypothesis with transgenic Brachypodium distachyon lines over-expressing endogenous RSL genes
文献类型：期刊论文
| 作者 | Zhang, Chunyan1,2,6; Simpson, Richard J.6; Kim, Chul Min3; Warthmann, Norman4; Delhaize, Emmanuel6; Dolan, Liam3; Byrne, Mary E.5; Wu, Yu1; Ryan, Peter R.6 |
| 刊名 | NEW PHYTOLOGIST
 |
| 出版日期 | 2018-03-01 |
| 卷号 | 217期号:4页码:1654-1666 |
| 关键词 | basic helix loop helix (bHLH) Brachypodium nutrition phosphorus (P) root hairs RSL transcription factor transgenic |
| ISSN号 | 0028-646X |
| DOI | 10.1111/nph.14980 |
| 产权排序 | 1 |
| 文献子类 | Article |
| 英文摘要 | Mutants without root hairs show reduced inorganic orthophosphate (Pi) uptake and compromised growth on soils when Pi availability is restricted. What is less clear is whether root hairs that are longer than wild-type provide an additional benefit to phosphorus (P) nutrition. This was tested using transgenic Brachypodium lines with longer root hairs. The lines were transformed with the endogenous BdRSL2 and BdRSL3 genes using either a constitutive promoter or a root hair-specific promoter. Plants were grown for 32 d in soil amended with various Pi concentrations. Plant biomass and P uptake were measured and genotypes were compared on the basis of critical Pi values and P uptake per unit root length. Ectopic expression of RSL2 and RSL3 increased root hair length three-fold but decreased plant biomass. Constitutive expression of BdRSL2, but not expression of BdRSL3, consistently improved P nutrition as measured by lowering the critical Pi values and increasing Pi uptake per unit root length. Increasing root hair length through breeding or biotechnology can improve P uptake efficiency if the pleotropic effects on plant biomass are avoided. Long root hairs, alone, appear to be insufficient to improve Pi uptake and need to be combined with other traits to benefit P nutrition. |
| 学科主题 | Plant Sciences |
| URL标识 | 查看原文 |
| WOS关键词 | PHOSPHATE STARVATION ; USE EFFICIENCY ; ACQUISITION EFFICIENCY ; ARABIDOPSIS-THALIANA ; PLANT-GROWTH ; SOIL ; LENGTH ; GENOTYPES ; GENOME ; TRAITS |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000424284400024 |
| 出版者 | WILEY |
| 源URL | [http://210.75.237.14/handle/351003/30324]  |
| 专题 | 环境治理与食品安全领域_农业生物技术研究 |
| 作者单位 | 1.Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 3.Univ Oxford, Dept Plant Sci, Oxford OX1 3RB, England; 4.Australian Natl Univ, Coll Med Biol & Environm, Canberra, ACT 2601, Australia; 5.Univ Sydney, Sch Life & Environm Sci, Sydney, NSW 2006, Australia 6.CSIRO Agr & Food, GPO Box 1700, Canberra, ACT 2601, Australia; |
| 推荐引用方式 GB/T 7714 | Zhang, Chunyan,Simpson, Richard J.,Kim, Chul Min,et al. Do longer root hairs improve phosphorus uptake? Testing the hypothesis with transgenic Brachypodium distachyon lines over-expressing endogenous RSL genes[J]. NEW PHYTOLOGIST,2018,217(4):1654-1666. |
| APA | Zhang, Chunyan.,Simpson, Richard J..,Kim, Chul Min.,Warthmann, Norman.,Delhaize, Emmanuel.,...&Ryan, Peter R..(2018).Do longer root hairs improve phosphorus uptake? Testing the hypothesis with transgenic Brachypodium distachyon lines over-expressing endogenous RSL genes.NEW PHYTOLOGIST,217(4),1654-1666. |
| MLA | Zhang, Chunyan,et al."Do longer root hairs improve phosphorus uptake? Testing the hypothesis with transgenic Brachypodium distachyon lines over-expressing endogenous RSL genes".NEW PHYTOLOGIST 217.4(2018):1654-1666. |
入库方式： OAI收割
来源：成都生物研究所
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