Analysis of DNA Methylation of Wheat in Response to Low Nitrogen Stress Based on Mathylation-Sensitive Amplified Polymorphisms
文献类型：期刊论文
| 作者 | Liao, Jinhua2; Zhou, Min1; Fan, Jin2; Hu, Xia2; Tang, Mei2; Gong, Mingfu2; Wu, Yu1 |
| 刊名 | INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY
 |
| 出版日期 | 2018-09-01 |
| 卷号 | 20期号:12页码:2897-2904 |
| 关键词 | DNA methylation/demethylation Gene expression Low nitrogen MSAP Wheat grain |
| ISSN号 | 1560-8530 |
| DOI | 10.16373/j.cnki.ahr.180039 |
| 产权排序 | 2 |
| 文献子类 | Article |
| 英文摘要 | Abiotic stress can induce considerable changes in the composition and quality of wheat grain and cause genomic DNA methylation. Here, methylation-sensitive amplified polymorphism (MSAP) was used to detect the effect of low nitrogen stress on the extent and patterns of DNA methylation in wheat grain at 5, 8, 11, 14, 16 and 21 days post-anthesis (DPA). First, our results showed that low nitrogen stress could reduce grain N content, sodium dodecylsulfate sedimentation (SDS) volume, the amounts of high-molecular-weight glutenin subunits (HMW-GS 2, 12 and 20) and total protein. Then, at 8, 11 and 21 DPA, the overall level of DNA methylation in low nitrogen group was higher than in control group with the maximum difference of 14.1% at 21 DPA. And, at 8 DPA, methylation / demethylation revealed the highest level under low nitrogen conditions, about 17.07% -30.99% of the CCGG sites. Thus, thirteen differentially displayed DNA fragments in MSAP profiles in samples at 8 DPA were further cloned for sequencing analysis. Homology analysis for these DNA fragments showed that most of them were involved in storage matter (protein, fatty acid), some engaged in stress signaling and physiological response regulation. Moreover, the relative expression levels revealed that the expression of a demethylated fragment was increased but the expressions of two other methylated fragments decreased. In conclusion, our results suggested that low nitrogen stress could decrease SDS volume, HMW-GS amount and total protein which might be associated with DNA methylation. (C) 2018 Friends Science Publishers |
| URL标识 | 查看原文 |
| WOS关键词 | BREAD-MAKING QUALITY ; DROUGHT ; TEMPERATURE ; SUBUNITS ; GLUTENIN ; ENVIRONMENT ; PROTEIN ; WATER ; SALT |
| WOS研究方向 | Agriculture ; Life Sciences & Biomedicine - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000454160600036 |
| 出版者 | FRIENDS SCIENCE PUBL |
| 源URL | [http://210.75.237.14/handle/351003/30356]  |
| 专题 | 环境治理与食品安全领域_农业生物技术研究 |
| 作者单位 | 1.Univ Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China 2.Leshan Normal Univ, Coll Life Sci, Leshan 614000, Sichuan, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Liao, Jinhua,Zhou, Min,Fan, Jin,et al. Analysis of DNA Methylation of Wheat in Response to Low Nitrogen Stress Based on Mathylation-Sensitive Amplified Polymorphisms[J]. INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY,2018,20(12):2897-2904. |
| APA | Liao, Jinhua.,Zhou, Min.,Fan, Jin.,Hu, Xia.,Tang, Mei.,...&Wu, Yu.(2018).Analysis of DNA Methylation of Wheat in Response to Low Nitrogen Stress Based on Mathylation-Sensitive Amplified Polymorphisms.INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY,20(12),2897-2904. |
| MLA | Liao, Jinhua,et al."Analysis of DNA Methylation of Wheat in Response to Low Nitrogen Stress Based on Mathylation-Sensitive Amplified Polymorphisms".INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY 20.12(2018):2897-2904. |
入库方式： OAI收割
来源：成都生物研究所
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