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Neutral Loss Scan - Based Strategy for Integrated Identification of Amorfrutin Derivatives, New Peroxisome Proliferator-Activated Receptor Gamma Agonists, from Amorpha Fruticosa by UPLC-QqQ-MS/MS and UPLC-Q-TOF-MS

文献类型：期刊论文

作者Chen, Chu2,3; Xue, Ying1; Li, Qing-Miao3; Wu, Yan3; Liang, Jian2,4; Qing, Lin-Sen4
刊名JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
出版日期2018-04-01
卷号29期号:4页码:685-693
关键词Amorfrutins PPAR gamma agonists Amorpha fruticosa UPLC-QqQ-MS/MS UPLC-Q-TOF-MS Neutral loss scan
ISSN号1044-0305
DOI10.1007/s13361-018-1891-4
产权排序1
文献子类Article
英文摘要Amorfrutins with a 2-hydroxybenzoic acid core structure are promising natural PPAR gamma agonists with potent antidiabetic activity. Owing to the complex matrix and low concentration in botanical material, the identification of unknown amorfrutins remains a challenge. In the present study, a combined application of UPLC-Q-TOF-MS and UPLC-QqQ-MS was developed to discover unknown amorfrutins from fruits of Amorpha fruticosa. First, reference compounds of amorfrutin A (AA), amorfrutin B (AB), and 2-carboxy-3,5-dihydroxy-4-geranylbibenzyl (AC) were analyzed using UPLC-Q-TOF-MS to reveal the characteristic fragment ions and the possible neutral loss. Second, the extract of A. fruticosa was separated and screened by UPLC-QqQ-MS using neutral loss scan to find out suspect compounds associated with the specified neutral fragment Delta m/z 44. Third, the extract was re-analyzed by UPLC-Q-TOF-MS to obtain the exact mass of quasi-molecular ion and fragment ions of each suspect compound, and to subsequently calculate their corresponding molecular formulas. Finally, according to the molecular formula of suspect compound and its fragment ions and comparing with literature data, structure elucidation of four unidentified amorfrutins was achieved. The results indicated that the combination of QqQ-MS neutral loss scan and Q-TOF-MS molecular formula calculation was proven to be a powerful tool for unknown natural product identification, and this strategy provides an effective solution to discover natural products or metabolites of trace content.
学科主题Spectroscopy ; Instrumentation ; Analytical Sciences
URL标识查看原文
WOS关键词TANDEM MASS-SPECTROMETRY ; LIQUID-CHROMATOGRAPHY ; ELECTROSPRAY-IONIZATION ; STRUCTURAL-CHARACTERIZATION ; NATURAL-PRODUCTS ; PPAR-GAMMA ; METABOLITES ; FLAVONOIDS ; GLYCOSIDES ; CHINENSIS
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy
语种英语
WOS记录号WOS:000429431900006
出版者SPRINGER
源URL[http://210.75.237.14/handle/351003/30284]  
专题国家天然药物工程技术研究中心_天然产物研究
生物多样性与生态系统服务领域_中国科学院山地生态恢复与生物资源利用重点实验室
生物多样性与生态系统服务领域_生态系统恢复与生物多样性保育四川省重点实验室
作者单位1.Sichuan Prov Ctr Dis Control & Prevent, Chengdu 610041, Sichuan, Peoples R China;
2.CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization and Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu,610041, China;
3.Sichuan Acad Chinese Med Sci, Chengdu 610041, Sichuan, Peoples R China;
4.Chinese Acad Sci, Chengdu Inst Biol, Nat Prod Res Ctr, Chengdu 610041, Sichuan, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Chu,Xue, Ying,Li, Qing-Miao,et al. Neutral Loss Scan - Based Strategy for Integrated Identification of Amorfrutin Derivatives, New Peroxisome Proliferator-Activated Receptor Gamma Agonists, from Amorpha Fruticosa by UPLC-QqQ-MS/MS and UPLC-Q-TOF-MS[J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,2018,29(4):685-693.
APA Chen, Chu,Xue, Ying,Li, Qing-Miao,Wu, Yan,Liang, Jian,&Qing, Lin-Sen.(2018).Neutral Loss Scan - Based Strategy for Integrated Identification of Amorfrutin Derivatives, New Peroxisome Proliferator-Activated Receptor Gamma Agonists, from Amorpha Fruticosa by UPLC-QqQ-MS/MS and UPLC-Q-TOF-MS.JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,29(4),685-693.
MLA Chen, Chu,et al."Neutral Loss Scan - Based Strategy for Integrated Identification of Amorfrutin Derivatives, New Peroxisome Proliferator-Activated Receptor Gamma Agonists, from Amorpha Fruticosa by UPLC-QqQ-MS/MS and UPLC-Q-TOF-MS".JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY 29.4(2018):685-693.

入库方式： OAI收割

来源：成都生物研究所

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