A novel PAX7 10-bp indel variant modulates promoter activity, gene expression and contributes to different phenotypes of Chinese cattle
文献类型：期刊论文
| 作者 | Xu, Yao1,3; Shi, Tao3; Zhou, Yang3; Liu, Mei3; Klaus, Sebastian2; Lan, Xianyong3; Lei, Chuzhao3; Chen, Hong3 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2019-01-29 |
| 卷号 | 8页码:1724 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/s41598-018-20177-8 |
| 产权排序 | 3 |
| 文献子类 | Article |
| 英文摘要 | Paired box 7 (PAX7) gene regulates the conversion of muscle satellite cells into myogenic cells and participates in multi-step processes in myogenesis. Expression levels of PAX7 are decisive for its regulatory function. Previous reports revealed that PAX7 were responsible for the developmental traits of muscle. The relationship of the PAX7 promoter variants and livestock phenotypic traits has not been fully elucidated. We detected a novel 10-bp insertion/deletion (indel) polymorphism in the bovine PAX7 promoter and revealed that the indel altered the binding of the transcriptional factor ZNF219. Luciferase reporter assay showed that deletion-deletion (Del-Del) genotype of the PAX7 gene showed 2.79-fold higher promoter activity than the insertion-insertion (Ins-Ins) genotype (P < 0.05), and ZNF219 overexpression significantly diminished the luciferase activity in Ins-Ins groups. Moreover, the expression of PAX7 and its down-stream genes were detected in fetal skeletal muscle of cattle with different PAX7 genotypes, where the Del-Del genotype also displayed high expression levels. Statistical association analysis demonstrated that this indel had significant effects on early growth traits in cattle. These findings provide a complete overview of the function of the PAX7 10-bp variant, which may have potential as a genetic marker for marker-assisted selection in improving economically significant traits of cattle. |
| 学科主题 | Multidisciplinary |
| URL标识 | 查看原文 |
| WOS关键词 | SATELLITE CELLS ; FUNCTIONAL-CHARACTERIZATION ; STEM-CELLS ; TRAITS ; GROWTH ; ZNF219 ; SNPS ; IDENTIFICATION ; ASSOCIATION ; MUTATIONS |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| 出版者 | NATURE PUBLISHING GROUP |
| WOS记录号 | WOS:000423428900012 |
| 源URL | [http://210.75.237.14/handle/351003/30349]  |
| 专题 | 生物多样性与生态系统服务领域_生态研究 |
| 作者单位 | 1.Wuhan Univ Sci & Technol, Coll Life Sci & Hlth, Inst Biol & Med, Wuhan 430081, Hubei, Peoples R China; 2.Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China 3.Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Xu, Yao,Shi, Tao,Zhou, Yang,et al. A novel PAX7 10-bp indel variant modulates promoter activity, gene expression and contributes to different phenotypes of Chinese cattle[J]. SCIENTIFIC REPORTS,2019,8:1724. |
| APA | Xu, Yao.,Shi, Tao.,Zhou, Yang.,Liu, Mei.,Klaus, Sebastian.,...&Chen, Hong.(2019).A novel PAX7 10-bp indel variant modulates promoter activity, gene expression and contributes to different phenotypes of Chinese cattle.SCIENTIFIC REPORTS,8,1724. |
| MLA | Xu, Yao,et al."A novel PAX7 10-bp indel variant modulates promoter activity, gene expression and contributes to different phenotypes of Chinese cattle".SCIENTIFIC REPORTS 8(2019):1724. |
入库方式： OAI收割
来源：成都生物研究所
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