中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Enhancement of osteogenesis using a novel porous hydroxyapatite scaffold in vivo and vitro

文献类型:期刊论文

作者Ren, Xiaohua2; Tuo, Qiang3; Tian, Kun2; Huang, Guo4; Li, Jinyu5; Xu, Taotao5; Lv, Xinrong6; Wu, Jia1; Chen, Zhenghui7; Weng, Jie5
刊名CERAMICS INTERNATIONAL
出版日期2019-12-01
卷号44期号:17页码:21656-21665
ISSN号0272-8842
关键词Bony defect Porous hydroxyapatite scaffolds Osteogenic
DOI10.1016/j.ceramint.2018.08.249
产权排序7
文献子类Article
英文摘要The repair of large maxillofacial bony defects using regular scaffolds is restricted by the osteogenic effect. It was postulated that a novel porous hydroxyapatite (HA) scaffolds with a 25-30 mu m groove structure (HAG) may counter this limitation. In this study we evaluated the biocompatibility of HAG scaffolds both in vitro and in vivo in beagle dogs by investigating the enhancement of scaffolds bioactivity and osteogenesis. Compared with a regular HA scaffolds, the HAG scaffolds significantly promoted human placenta-derived mesenchymal stem cell (hPMSC) osteogenic differentiation and the maturation of osteoblasts. This is achieved by increasing protein adsorption, as well as promoting directed growth and expression of osteogenic genes in vitro. The compressive strength of HAG scaffolds was significantly greater than HA in both dorsal muscle and the mandibular distraction area after in vivo implantation, with hematoxylin and eosin staining demonstrating new bone formation and vasculogenesis. Immunochemical staining and micro-CT scanning demonstrated increased expression of osteogenic factors (BMP2, OCN and COL-1) and bone density in the HAG scaffolds compared with HA. Based on the above results, we conclude that HAG scaffolds that have a groove structure induce greater osteogenesis and possess improved ostoegenesis which could be utilized in the clinical treatment.
学科主题Applied Physics/condensed Matter/materials Science
URL标识查看原文
WOS关键词MESENCHYMAL STEM-CELLS ; BONE TISSUE ; BLOOD-VESSELS ; STROMAL CELLS ; PORE-SIZE ; REGENERATION ; SURFACE ; MODEL ; VASCULARIZATION ; OSTEOBLASTS
WOS研究方向Materials Science
语种英语
出版者ELSEVIER SCI LTD
WOS记录号WOS:000448226900135
源URL[http://210.75.237.14/handle/351003/30089]  
专题国家天然药物工程技术研究中心_天然产物研究
作者单位1.Department of Stomatology, Nuclear Industry 416 Hospital, China;
2.Department of Stomatology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, China;
3.University of Electronic Science and Technology of China, China;
4.The Sichuan Provincial Key Laboratory for Human Disease Gene Study, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, School of Medicine,University of Electronic Science and Technology of China, China;
5.Key Laboratory of Advanced Technologies of Materials (Ministry of Education), School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu,Sichuan 610031,China;
6.Southwest Medical University School of Stomatolog, China;
7.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
推荐引用方式
GB/T 7714
Ren, Xiaohua,Tuo, Qiang,Tian, Kun,et al. Enhancement of osteogenesis using a novel porous hydroxyapatite scaffold in vivo and vitro[J]. CERAMICS INTERNATIONAL,2019,44(17):21656-21665.
APA Ren, Xiaohua.,Tuo, Qiang.,Tian, Kun.,Huang, Guo.,Li, Jinyu.,...&Mu, Yandong.(2019).Enhancement of osteogenesis using a novel porous hydroxyapatite scaffold in vivo and vitro.CERAMICS INTERNATIONAL,44(17),21656-21665.
MLA Ren, Xiaohua,et al."Enhancement of osteogenesis using a novel porous hydroxyapatite scaffold in vivo and vitro".CERAMICS INTERNATIONAL 44.17(2019):21656-21665.

入库方式: OAI收割

来源:成都生物研究所

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