Simultaneous determination of trace Aflatoxin B-1 and Ochratoxin A by aptamer-based microchip capillary electrophoresis in food samples
文献类型：期刊论文
| 作者 | Xiao, Meng-Wei1,3; Bai, Xiao-Lin1; Liu, Yi-Ming1,4; Yang, Li2; Liao, Xun1 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2019-09-28 |
| 卷号 | 1569期号:2018页码:222-228 |
| 关键词 | Microchip capillary electrophoresis Laser induced fluorescence Aptamer Aflatoxin B-1 Ochratoxin A |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2018.07.051 |
| 产权排序 | 1 |
| 文献子类 | Article |
| 英文摘要 | An aptamer-based microchip capillary electrophoresis coupled with laser induced fluorescence (MCE-LIF) detection method for fast determination of Aflatoxin B-1 (AFB(1)) and Ochratoxin A (OTA) was developed. Aptamers that are specific to these two mycotoxins were first hybridized with their aptamer complementary oligonucleotides. The double strand DNA that comes in contact with mycotoxin-containing environment would be unwound into separate aptamer-mycotoxin complex and aptamer complementary single strand. Different types of oligonucleotides can be separated in MCE and detected under the aid of fluorescent dye SYBR gold in LIF detection unit. Under the optimal conditions, on-chip aptamer-mycotoxin conjugates analysis was achieved within 3 min with extremely low LODs (0.026 ng/mL for AFB(1) and 0.021 ng/mL for OTA). Specificity study indicated that other major mycotoxins would not cross-react with these two aptamers, demonstrating the good selectivity of the proposed method. Quantification of trace AFB(1) and OTA in real food samples was carried out and satisfactory recoveries were obtained. It is demonstrated that this method is fast, facile and specific for Simultaneous determination of trace AFB(1) and OTA from foodstuffs. (C) 2018 Elsevier B.V. All rights reserved. |
| 学科主题 | Chemistry ; Analysis |
| URL标识 | 查看原文 |
| WOS关键词 | POLYMERASE-CHAIN-REACTION ; SYBR GOLD ; CHROMATOGRAPHY ; MYCOTOXINS ; EXTRACTION ; BIOSENSOR ; SELECTION ; ASSAY ; G(1) ; G(2) |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000442973200025 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://210.75.237.14/handle/351003/30150]  |
| 专题 | 国家天然药物工程技术研究中心_天然产物研究 |
| 作者单位 | 1.Chinese Acad Sci, Chengdu Inst Biol, 9,Sect 4,South Renmin Rd, Chengdu, Sichuan, Peoples R China; 2.Maccura Biotechnol Co Ltd, 2nd Anhe Rd, Chengdu, Sichuan, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China; 4.Jackson State Univ, Dept Chem & Biochem, 1400 Lynch St, Jackson, MS 39217 USA; |
| 推荐引用方式 GB/T 7714 | Xiao, Meng-Wei,Bai, Xiao-Lin,Liu, Yi-Ming,et al. Simultaneous determination of trace Aflatoxin B-1 and Ochratoxin A by aptamer-based microchip capillary electrophoresis in food samples[J]. JOURNAL OF CHROMATOGRAPHY A,2019,1569(2018):222-228. |
| APA | Xiao, Meng-Wei,Bai, Xiao-Lin,Liu, Yi-Ming,Yang, Li,&Liao, Xun.(2019).Simultaneous determination of trace Aflatoxin B-1 and Ochratoxin A by aptamer-based microchip capillary electrophoresis in food samples.JOURNAL OF CHROMATOGRAPHY A,1569(2018),222-228. |
| MLA | Xiao, Meng-Wei,et al."Simultaneous determination of trace Aflatoxin B-1 and Ochratoxin A by aptamer-based microchip capillary electrophoresis in food samples".JOURNAL OF CHROMATOGRAPHY A 1569.2018(2019):222-228. |
入库方式： OAI收割
来源：成都生物研究所
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