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Profiling the origin, dynamics, and function of traction force in B cell activation

文献类型：期刊论文

作者Wang, Junyi5; Lin, Feng4; Wan, Zhengpeng5; Sun, Xiaolin3; Lu, Yun10; Huang, Jianyong4; Wang, Fei6; Zeng, Yingyue7; Chen, Ying-Hua5; Shi, Yan8
刊名SCIENCE SIGNALING
出版日期2019-08-07
卷号11期号:542页码:eaai9192
ISSN号1945-0877
DOI10.1126/scisignal.aai9192
产权排序5
文献子类Article
英文摘要B lymphocytes use B cell receptors (BCRs) to recognize membrane-bound antigens to further initiate cell spreading and contraction responses during B cell activation. We combined traction force microscopy and live-cell imaging to profile the origin, dynamics, and function of traction force generation in these responses. We showed that B cell activation required the generation of 10 to 20 nN of traction force when encountering antigens presented by substrates with stiffness values from 0.5 to 1 kPa, which mimic the rigidity of antigen-presenting cells in vivo. Perturbation experiments revealed that F-actin remodeling and myosin- and dynein-mediated contractility contributed to traction force generation and B cell activation. Moreover, membrane-proximal BCR signaling molecules (including Lyn, Syk, Btk, PLC-gamma 2, BLNK, and Vav3) and adaptor molecules (Grb2, Cbl, and Dok-3) linking BCR microclusters and motor proteins were also required for the sustained generation of these traction forces. We found a positive correlation between the strength of the traction force and the mean fluorescence intensity of the BCR microclusters. Furthermore, we demonstrated that isotype-switched memory B cells expressing immunoglobulin G (IgG)-BCRs generated greater traction forces than did mature naive B cells expressing IgM-BCRs during B cell activation. Last, we observed that primary B cells from patients with rheumatoid arthritis generated greater traction forces than did B cells from healthy donors in response to antigen stimulation. Together, these data delineate the origin, dynamics, and function of traction force during B cell activation.
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WOS关键词HIGH EPITOPE DENSITY ; MECHANICAL-PROPERTIES ; INTEGRIN ACTIVATION ; PROTEIN MOLECULE ; T-CELLS ; ADHESION ; ACTIN ; TAIL ; IGG ; STIFFNESS
WOS研究方向Biochemistry & Molecular Biology ; Cell Biology
语种英语
出版者AMER ASSOC ADVANCEMENT SCIENCE
WOS记录号WOS:000440925500001
源URL[http://210.75.237.14/handle/351003/30186]  
专题国家天然药物工程技术研究中心_天然产物研究
作者单位1.Beijing Key Lab Immunol Res Chron Dis, Beijing 100084, Peoples R China
2.Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China;
3.Peking Univ, Peoples Hosp, Dept Rheumatol & Immunol, Beijing 100871, Peoples R China;
4.Peking Univ, Coll Engn, Dept Mech & Engn Sci, Beijing 100871, Peoples R China;
5.Tsinghua Univ, Collaborat Innovat Ctr Diag & Treatment Infect Di, China Minist Educ, Key Lab Prot Sci,Sch Life Sci,Inst Immunol, Beijing 100084, Peoples R China;
6.Chinese Acad Sci, Chengdu Inst Biol, 9 Sect 4,Renmin South Rd, Chengdu 610041, Sichuan, Peoples R China;
7.Liaoning Univ, Sch Life Sci, Shenyang 110036, Liaoning, Peoples R China;
8.Tsinghua Univ, Ctr Life Sci, Inst Immunol, Dept Basic Med Sci, Beijing 100084, Peoples R China;
9.Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, China.;
10.Tsinghua Univ, Sch Environm, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100084, Peoples R China;
推荐引用方式
GB/T 7714		 Wang, Junyi,Lin, Feng,Wan, Zhengpeng,et al. Profiling the origin, dynamics, and function of traction force in B cell activation[J]. SCIENCE SIGNALING,2019,11(542):eaai9192.
APA Wang, Junyi.,Lin, Feng.,Wan, Zhengpeng.,Sun, Xiaolin.,Lu, Yun.,...&Liu, Wanli.(2019).Profiling the origin, dynamics, and function of traction force in B cell activation.SCIENCE SIGNALING,11(542),eaai9192.
MLA Wang, Junyi,et al."Profiling the origin, dynamics, and function of traction force in B cell activation".SCIENCE SIGNALING 11.542(2019):eaai9192.

入库方式： OAI收割

来源：成都生物研究所

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