PI(4,5)P2 determines the threshold of mechanical force-induced B cell activation
文献类型：期刊论文
| 作者 | Wan, Zhengpeng2; Xu, Chenguang2; Chen, Xiangjun2; Xie, Hengyi2; Li, Zongyu2; Wang, Jing2; Ji, Xingyu2; Chen, Haodong3; Ji, Qinghua4; Shaheen, Samina2 |
| 刊名 | JOURNAL OF CELL BIOLOGY
 |
| 出版日期 | 2018-07-01 |
| 卷号 | 217期号:7页码:2565-2582 |
| ISSN号 | 0021-9525 |
| DOI | 10.1083/jcb.201711055 |
| 产权排序 | 2 |
| 文献子类 | Article |
| 英文摘要 | B lymphocytes use B cell receptors (BCRs) to sense the chemical and physical features of antigens. The activation of isotype-switched IgG-BCR by mechanical force exhibits a distinct sensitivity and threshold in comparison with IgM-BCR. However, molecular mechanisms governing these differences remain to be identified. In this study, we report that the low threshold of IgG-BCR activation by mechanical force is highly dependent on tethering of the cytoplasmic tail of the IgG-BCR heavy chain (IgG-tail)to the plasma membrane. Mechanistically, we show that the positively charged residues in the IgG-tail play a crucial role by highly enriching phosphatidylinositol (4,5)-biphosphate (PI(4,5)P2)into the membrane microdomains of IgG-BCRs. Indeed, manipulating the amounts of PI(4,5)P2 within IgG-BCR membrane microdomains significantly altered the threshold and sensitivity of IgG-BCR activation. Our results reveal a lipid-dependent mechanism for determining the threshold of IgG-BCR activation by mechanical force. |
| 学科主题 | Cell ; Developmental Biology |
| URL标识 | 查看原文 |
| WOS关键词 | HIGH EPITOPE DENSITY ; ANTIGEN RECEPTOR ; PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE ; SIGNALING MOLECULES ; PHOSPHATIDIC-ACID ; PROTEIN MOLECULE ; LIPID RAFTS ; SURFACE ; MEMORY ; ACTIN |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000438081900028 |
| 出版者 | ROCKEFELLER UNIV PRESS |
| 源URL | [http://210.75.237.14/handle/351003/30217]  |
| 专题 | 国家天然药物工程技术研究中心_天然产物研究 |
| 作者单位 | 1.Zhejiang Univ, Sch Basic Med Sci, Hangzhou, Zhejiang, Peoples R China; 2.Tsinghua Univ, Inst Immunol, Sch Life Sci,Minist Educ,Key Lab Prot Sci, Collaborat Innovat Ctr Diag & Treatment Infect Di, Beijing, Peoples R China; 3.Chinese Acad Sci, Chengdu Inst Biol, Natl Prod Res Ctr, Chengdu, Sichuan, Peoples R China; 4.Chinese Acad Sci, Lab RNA Biol, Inst Biophys, Beijing, Peoples R China; 5.Univ Sci & Technol China, Sch Life Sci, Hefei, Anhui, Peoples R China; 6.Beijing Key Lab Immunol Res Chron Dis, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wan, Zhengpeng,Xu, Chenguang,Chen, Xiangjun,et al. PI(4,5)P2 determines the threshold of mechanical force-induced B cell activation[J]. JOURNAL OF CELL BIOLOGY,2018,217(7):2565-2582. |
| APA | Wan, Zhengpeng.,Xu, Chenguang.,Chen, Xiangjun.,Xie, Hengyi.,Li, Zongyu.,...&Liu, Wanli.(2018).PI(4,5)P2 determines the threshold of mechanical force-induced B cell activation.JOURNAL OF CELL BIOLOGY,217(7),2565-2582. |
| MLA | Wan, Zhengpeng,et al."PI(4,5)P2 determines the threshold of mechanical force-induced B cell activation".JOURNAL OF CELL BIOLOGY 217.7(2018):2565-2582. |
入库方式： OAI收割
来源：成都生物研究所
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